Transcriptional regulation of latent feline immunodeficiency virus in peripheral CD4+ T-lymphocytes

Samantha J. McDonnel, Ellen E. Sparger, Paul A Luciw, Brian G Murphy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Feline immunodeficiency virus (FIV), the lentivirus of domestic cats responsible for feline AIDS, establishes a latent infection in peripheral blood CD4+ T-cells approximately eight months after experimental inoculation. In this study, cats experimentally infected with the FIV-C strain in the asymptomatic phase demonstrated an estimated viral load of 1 infected cell per approximately 103 CD4+ T-cells, with about 1 copy of viral DNA per cell. Approximately 1 in 10 proviral copies was capable of transcription in the asymptomatic phase. The latent FIV proviral promoter was associated with deacetylated, methylated histones, which is consistent with a condensed chromatin structure. In contrast, the transcriptionally active FIV promoter was associated with histone acetylation and demethylation. In addition, RNA polymerase II appeared to be paused on the latent viral promoter, and short promoter-proximal transcripts were detected. Our findings for the FIV promoter in infected cats are similar to results obtained in studies of human immunodeficiency virus (HIV)-1 latent proviruses in cell culture in vitro studies. Thus, the FIV/cat model may offer insights into in vivo mechanisms of HIV latency and provides a unique opportunity to test novel therapeutic interventions aimed at eradicating latent virus.

Original languageEnglish (US)
Pages (from-to)878-888
Number of pages11
JournalViruses
Volume4
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Feline Immunodeficiency Virus
T-Lymphocytes
Cats
Feline Acquired Immunodeficiency Syndrome
Histones
Virus Latency
Proviruses
Lentivirus
RNA Polymerase II
Viral DNA
Acetylation
Viral Load
Chromatin
HIV-1
Cell Culture Techniques
HIV
Viruses
Infection

Keywords

  • CD4 T-cells
  • Chromatin
  • FIV
  • Latency
  • Lentivirus
  • Transcription

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Transcriptional regulation of latent feline immunodeficiency virus in peripheral CD4+ T-lymphocytes. / McDonnel, Samantha J.; Sparger, Ellen E.; Luciw, Paul A; Murphy, Brian G.

In: Viruses, Vol. 4, No. 5, 05.2012, p. 878-888.

Research output: Contribution to journalArticle

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