Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex

Kartik Pattabiraman, Olga Golonzhka, Susan Lindtner, Alexander Nord, Leila Taher, Renee Hoch, Shanni N. Silberberg, Dongji Zhang, Bin Chen, Hong Kui Zeng, Len A. Pennacchio, Luis Puelles, Axel Visel, John L.R. Rubenstein

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Elucidating the genetic control of cerebral cortical (pallial) development is essential for understanding function, evolution, and disorders of the brain. Transcription factors (TFs) that embryonically regulate pallial regionalization are expressed in gradients, raising the question of how discrete domains are generated. We provide evidence that small enhancer elements active in protodomains integrate broad transcriptional information. CreERT2 and GFP expression from 14 different enhancer elements in stable transgenic mice allowed us to define a comprehensive regional fate map of the pallium. We explored transcriptional mechanisms that control the activity of the enhancers using informatics, invivo occupancy by TFs that regulate cortical patterning (CoupTFI, Pax6, and Pbx1), and analysis of enhancer activity in Pax6 mutants. Overall, the results provide insights into how broadly expressed patterning TFs regulate the activity of small enhancer elements that drive gene expression in pallial protodomains that fate map to distinct cortical regions.

Original languageEnglish (US)
Pages (from-to)989-1003
Number of pages15
JournalNeuron
Volume82
Issue number5
DOIs
StatePublished - Jun 4 2014
Externally publishedYes

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Cerebral Cortex
Transcription Factors
Informatics
Brain Diseases
Transgenic Mice
Gene Expression

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pattabiraman, K., Golonzhka, O., Lindtner, S., Nord, A., Taher, L., Hoch, R., ... Rubenstein, J. L. R. (2014). Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex. Neuron, 82(5), 989-1003. https://doi.org/10.1016/j.neuron.2014.04.014

Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex. / Pattabiraman, Kartik; Golonzhka, Olga; Lindtner, Susan; Nord, Alexander; Taher, Leila; Hoch, Renee; Silberberg, Shanni N.; Zhang, Dongji; Chen, Bin; Zeng, Hong Kui; Pennacchio, Len A.; Puelles, Luis; Visel, Axel; Rubenstein, John L.R.

In: Neuron, Vol. 82, No. 5, 04.06.2014, p. 989-1003.

Research output: Contribution to journalArticle

Pattabiraman, K, Golonzhka, O, Lindtner, S, Nord, A, Taher, L, Hoch, R, Silberberg, SN, Zhang, D, Chen, B, Zeng, HK, Pennacchio, LA, Puelles, L, Visel, A & Rubenstein, JLR 2014, 'Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex', Neuron, vol. 82, no. 5, pp. 989-1003. https://doi.org/10.1016/j.neuron.2014.04.014
Pattabiraman, Kartik ; Golonzhka, Olga ; Lindtner, Susan ; Nord, Alexander ; Taher, Leila ; Hoch, Renee ; Silberberg, Shanni N. ; Zhang, Dongji ; Chen, Bin ; Zeng, Hong Kui ; Pennacchio, Len A. ; Puelles, Luis ; Visel, Axel ; Rubenstein, John L.R. / Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex. In: Neuron. 2014 ; Vol. 82, No. 5. pp. 989-1003.
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