TY - JOUR
T1 - Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex
AU - Pattabiraman, Kartik
AU - Golonzhka, Olga
AU - Lindtner, Susan
AU - Nord, Alexander
AU - Taher, Leila
AU - Hoch, Renee
AU - Silberberg, Shanni N.
AU - Zhang, Dongji
AU - Chen, Bin
AU - Zeng, Hong Kui
AU - Pennacchio, Len A.
AU - Puelles, Luis
AU - Visel, Axel
AU - Rubenstein, John L.R.
PY - 2014/6/4
Y1 - 2014/6/4
N2 - Elucidating the genetic control of cerebral cortical (pallial) development is essential for understanding function, evolution, and disorders of the brain. Transcription factors (TFs) that embryonically regulate pallial regionalization are expressed in gradients, raising the question of how discrete domains are generated. We provide evidence that small enhancer elements active in protodomains integrate broad transcriptional information. CreERT2 and GFP expression from 14 different enhancer elements in stable transgenic mice allowed us to define a comprehensive regional fate map of the pallium. We explored transcriptional mechanisms that control the activity of the enhancers using informatics, invivo occupancy by TFs that regulate cortical patterning (CoupTFI, Pax6, and Pbx1), and analysis of enhancer activity in Pax6 mutants. Overall, the results provide insights into how broadly expressed patterning TFs regulate the activity of small enhancer elements that drive gene expression in pallial protodomains that fate map to distinct cortical regions.
AB - Elucidating the genetic control of cerebral cortical (pallial) development is essential for understanding function, evolution, and disorders of the brain. Transcription factors (TFs) that embryonically regulate pallial regionalization are expressed in gradients, raising the question of how discrete domains are generated. We provide evidence that small enhancer elements active in protodomains integrate broad transcriptional information. CreERT2 and GFP expression from 14 different enhancer elements in stable transgenic mice allowed us to define a comprehensive regional fate map of the pallium. We explored transcriptional mechanisms that control the activity of the enhancers using informatics, invivo occupancy by TFs that regulate cortical patterning (CoupTFI, Pax6, and Pbx1), and analysis of enhancer activity in Pax6 mutants. Overall, the results provide insights into how broadly expressed patterning TFs regulate the activity of small enhancer elements that drive gene expression in pallial protodomains that fate map to distinct cortical regions.
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U2 - 10.1016/j.neuron.2014.04.014
DO - 10.1016/j.neuron.2014.04.014
M3 - Article
C2 - 24814534
AN - SCOPUS:84901843699
VL - 82
SP - 989
EP - 1003
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 5
ER -