Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons

Magnus Sandberg, Pierre Flandin, Shanni Silberberg, Linda Su-Feher, James D. Price, Jia Sheng Hu, Carol Kim, Axel Visel, Alexander Nord, John L.R. Rubenstein

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The embryonic basal ganglia generates multiple projection neurons and interneuron subtypes from distinct progenitor domains. Combinatorial interactions of transcription factors and chromatin are thought to regulate gene expression. In the medial ganglionic eminence, the NKX2-1 transcription factor controls regional identity and, with LHX6, is necessary to specify pallidal projection neurons and forebrain interneurons. Here, we dissected the molecular functions of NKX2-1 by defining its chromosomal binding, regulation of gene expression, and epigenetic state. NKX2-1 binding at distal regulatory elements led to a repressed epigenetic state and transcriptional repression in the ventricular zone. Conversely, NKX2-1 is required to establish a permissive chromatin state and transcriptional activation in the sub-ventricular and mantle zones. Moreover, combinatorial binding of NKX2-1 and LHX6 promotes transcriptionally permissive chromatin and activates genes expressed in cortical migrating interneurons. Our integrated approach provides a foundation for elucidating transcriptional networks guiding the development of the MGE and its descendants.

Original languageEnglish (US)
Pages (from-to)1260-1275
Number of pages16
JournalNeuron
Volume91
Issue number6
DOIs
StatePublished - Sep 21 2016

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GABAergic Neurons
Gene Regulatory Networks
Interneurons
Prosencephalon
Chromatin
Epigenomics
Transcription Factors
Neurons
Median Eminence
Gene Expression Regulation
Basal Ganglia
Transcriptional Activation
Gene Expression
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sandberg, M., Flandin, P., Silberberg, S., Su-Feher, L., Price, J. D., Hu, J. S., ... Rubenstein, J. L. R. (2016). Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. Neuron, 91(6), 1260-1275. https://doi.org/10.1016/j.neuron.2016.08.020

Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. / Sandberg, Magnus; Flandin, Pierre; Silberberg, Shanni; Su-Feher, Linda; Price, James D.; Hu, Jia Sheng; Kim, Carol; Visel, Axel; Nord, Alexander; Rubenstein, John L.R.

In: Neuron, Vol. 91, No. 6, 21.09.2016, p. 1260-1275.

Research output: Contribution to journalArticle

Sandberg, M, Flandin, P, Silberberg, S, Su-Feher, L, Price, JD, Hu, JS, Kim, C, Visel, A, Nord, A & Rubenstein, JLR 2016, 'Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons', Neuron, vol. 91, no. 6, pp. 1260-1275. https://doi.org/10.1016/j.neuron.2016.08.020
Sandberg M, Flandin P, Silberberg S, Su-Feher L, Price JD, Hu JS et al. Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. Neuron. 2016 Sep 21;91(6):1260-1275. https://doi.org/10.1016/j.neuron.2016.08.020
Sandberg, Magnus ; Flandin, Pierre ; Silberberg, Shanni ; Su-Feher, Linda ; Price, James D. ; Hu, Jia Sheng ; Kim, Carol ; Visel, Axel ; Nord, Alexander ; Rubenstein, John L.R. / Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. In: Neuron. 2016 ; Vol. 91, No. 6. pp. 1260-1275.
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