Transcriptional modulation of viral reporter gene constructs following induction of the cellular stress response

Janice M. Andrews, Garret C. Newbound, Michael Dale Lairmore

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

In this study, we report that commonly used methods of transient transfection induce the cellular stress response and a recovery period is required following transfection when analyzing cellular stress responsive genes. Four transfection methods were examined for their ability to induce the stress response by measuring the expression of heat shock protein (hsp) 72. We demonstrate that electroporation increases expression of hsp 72 in HUT 78 cells. Additionally, DEAE-dextran and liposome-mediated transfection resulted in increased hsp 72 expression in an adherent cell line (HeLa). Liposome-mediated transfection differentially induced cell stress, dependent on the transfection time in serum-free culture conditions. The stress responsiveness of two viral promoters, the HTLV-1 long terminal repeat and CMV immediate early transcriptional unit were examined. We found the maximal stress-mediated enhancement of transcription with both promoters did not occur until the cells recovered for 24 h following transfection.

Original languageEnglish (US)
Pages (from-to)1082-1084
Number of pages3
JournalNucleic Acids Research
Volume25
Issue number5
DOIs
StatePublished - Mar 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Transcriptional modulation of viral reporter gene constructs following induction of the cellular stress response'. Together they form a unique fingerprint.

Cite this