Transcriptional complexity of the Anopheles stephensi nitric oxide synthase gene

S. Luckhart, K. Li

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Anopheles stephensi nitric oxide synthase (AsNOS) is a single copy gene that shares significant structural homology with the three human NOS genes and is inducibly expressed in Plasmodium-infected mosquitoes. Exon-specific Northern analyses and exon-spanning polymerase chain reaction amplification were used to further characterize transcription from this gene. A total of 18-22 AsNOS transcripts, ranging in size from 1.0 to 7.5 kb, were detected in replicated Northern blots from three separate cohorts of mosquitoes. Three transcripts (1604, 2330, and 2585 bp) were significantly induced in Plasmodium-infected mosquitoes (p<0.05), while others showed varying patterns of induction or downregulation. Five splice variants contained deletions of 1-7 exons. All but one deletion pattern was predicted to introduce in-frame stop codons or alter the translational reading frame. A novel insertion derived from intron sequence was predicted to introduce in-frame stop codons following exon 11. Two truncated novel exon 1 variants were identified that are homologous to a previously published 5′ sequence for this exon. The large number of AsNOS transcripts and diversity in AsNOS splicing and exon 1 sequences indicate that transcriptional complexity is a hallmark of both invertebrate and vertebrate NOS genes.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume31
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Anopheles stephensi
Anopheles
nitric oxide synthase
Nitric Oxide Synthase
exons
Exons
Genes
Culicidae
genes
Plasmodium
Terminator Codon
stop codon
Reading Frames
Polymerase chain reaction
Invertebrates
Transcription
Northern blotting
Northern Blotting
Introns
Amplification

Keywords

  • Anopheles
  • Insect immunity
  • Malaria
  • Mosquito
  • Nitric oxide synthase
  • Plasmodium
  • Transcription

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Transcriptional complexity of the Anopheles stephensi nitric oxide synthase gene. / Luckhart, S.; Li, K.

In: Insect Biochemistry and Molecular Biology, Vol. 31, No. 3, 2001, p. 249-256.

Research output: Contribution to journalArticle

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