Transcription profiling reveals potential mechanisms of dysbiosis in the oral microbiome of rhesus macaques with chronic untreated SIV infection

Susan Ocon, Christina Murphy, Angeline T. Dang, Sumathi Sankaran-Walters, Chin-Shang Li, Ross Tarara, Niku Borujerdpur, Satya Dandekar, Bruce J. Paster, Michael D. George

Research output: Contribution to journalArticle

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Abstract

A majority of individuals infected with human immunodeficiency virus (HIV) have inadequate access to antiretroviral therapy and ultimately develop debilitating oral infections that often correlate with disease progression. Due to the impracticalities of conducting host-microbe systems-based studies in HIV infected patients, we have evaluated the potential of simian immunodeficiency virus (SIV) infected rhesus macaques to serve as a non-human primate model for oral manifestations of HIV disease. We present the first description of the rhesus macaque oral microbiota and show that a mixture of human commensal bacteria and "macaque versions" of human commensals colonize the tongue dorsum and dental plaque. Our findings indicate that SIV infection results in chronic activation of antiviral and inflammatory responses in the tongue mucosa that may collectively lead to repression of epithelial development and impact the microbiome. In addition, we show that dysbiosis of the lingual microbiome in SIV infection is characterized by outgrowth of Gemella morbillorum that may result from impaired macrophage function. Finally, we provide evidence that the increased capacity of opportunistic pathogens (e.g. E. coli) to colonize the microbiome is associated with reduced production of antimicrobial peptides.

Original languageEnglish (US)
Article numbere80863
JournalPLoS One
Volume8
Issue number11
DOIs
StatePublished - Nov 29 2013

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Dysbiosis
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Microbiota
Virus Diseases
Transcription
Macaca mulatta
Viruses
mouth
Human immunodeficiency virus
tongue
Tongue
HIV
Gemella morbillorum
infection
Gemella
Oral Manifestations
Dental Plaque
antimicrobial peptides
Macaca

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Transcription profiling reveals potential mechanisms of dysbiosis in the oral microbiome of rhesus macaques with chronic untreated SIV infection. / Ocon, Susan; Murphy, Christina; Dang, Angeline T.; Sankaran-Walters, Sumathi; Li, Chin-Shang; Tarara, Ross; Borujerdpur, Niku; Dandekar, Satya; Paster, Bruce J.; George, Michael D.

In: PLoS One, Vol. 8, No. 11, e80863, 29.11.2013.

Research output: Contribution to journalArticle

Ocon, Susan ; Murphy, Christina ; Dang, Angeline T. ; Sankaran-Walters, Sumathi ; Li, Chin-Shang ; Tarara, Ross ; Borujerdpur, Niku ; Dandekar, Satya ; Paster, Bruce J. ; George, Michael D. / Transcription profiling reveals potential mechanisms of dysbiosis in the oral microbiome of rhesus macaques with chronic untreated SIV infection. In: PLoS One. 2013 ; Vol. 8, No. 11.
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