Transcription profile of Helicobacter pylori in the human stomach reflects its physiology in vivo

Jenni K. Boonjakuakul, Michael Syvanen, Arun Suryaprasad, Christopher Bowlus, Jay V Solnick

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. Little is known about levels of expression of Helicobacter pylori genes in the human host. We therefore developed a quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) assay to measure transcript profiles of H. pylori in the human stomach. Methods. In vivo expression of 16 genes on the cag pathogenicity island and of 18 putative virulence genes was quantitated by isolation of total RNA directly from infected human gastric mucosa. The results were compared with in vitro expression determined from H. pylori cells grown in culture. Results. The highest levels of expression were found for cag1 and cag25 and for genes, such as urease and catalase, that may be important for bacterial homeostasis in the relatively hostile environment of the gastric mucosa. Transcript abundance, relative to 16S rRNA, was lower in vivo than in vitro, which suggests that H. pylori cells are in stationary phase in the gastric environment. This was particularly apparent for cagA. Since CagA is arguably of unique importance, in terms of interaction with the host, tight control of its in vivo expression might be particularly important. Conclusions. qRT-PCR is a powerful tool to measure gene expression in human or animal tissue that contains minute amounts of microbial mRNA, and the results reflect on the physiology of the pathogen in its natural host.

Original languageEnglish (US)
Pages (from-to)946-956
Number of pages11
JournalJournal of Infectious Diseases
Volume190
Issue number5
DOIs
StatePublished - Sep 1 2004

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Helicobacter pylori
Stomach
Gastric Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Genes
Genomic Islands
Gene Expression
Urease
Catalase
Virulence
Homeostasis
RNA
Messenger RNA
In Vitro Techniques

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Transcription profile of Helicobacter pylori in the human stomach reflects its physiology in vivo. / Boonjakuakul, Jenni K.; Syvanen, Michael; Suryaprasad, Arun; Bowlus, Christopher; Solnick, Jay V.

In: Journal of Infectious Diseases, Vol. 190, No. 5, 01.09.2004, p. 946-956.

Research output: Contribution to journalArticle

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