Transcription of killer virion double-stranded RNA in vitro

J. Douglas Welsh; Michael J Leibowitz

doi:10.1093/nar/8.11.2365

Transcription of killer virion double-stranded RNA in vitro

J. Douglas Welsh, Michael J Leibowitz

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 10⁶ dalton) and L (3.2 × 10⁶ dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

Original language	English (US)
Pages (from-to)	2365-2376
Number of pages	12
Journal	Nucleic Acids Research
Volume	8
Issue number	11
DOIs	https://doi.org/10.1093/nar/8.11.2365
State	Published - Jun 11 1980
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Health, Toxicology and Mutagenesis
Toxicology
Genetics(clinical)
Genetics

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AB - Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

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Transcription of killer virion double-stranded RNA in vitro

Abstract

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