Abstract
Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2365-2376 |
| Number of pages | 12 |
| Journal | Nucleic Acids Research |
| Volume | 8 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 11 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
Transcription of killer virion double-stranded RNA in vitro. / Welsh, J. Douglas; Leibowitz, Michael J.
In: Nucleic Acids Research, Vol. 8, No. 11, 11.06.1980, p. 2365-2376.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcription of killer virion double-stranded RNA in vitro
AU - Welsh, J. Douglas
AU - Leibowitz, Michael J
PY - 1980/6/11
Y1 - 1980/6/11
N2 - Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.
AB - Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.
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U2 - 10.1093/nar/8.11.2365
DO - 10.1093/nar/8.11.2365
M3 - Article
C2 - 7003534
AN - SCOPUS:0019322275
VL - 8
SP - 2365
EP - 2376
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 11
ER -