Transcription of killer virion double-stranded RNA in vitro

J. Douglas Welsh, Michael J Leibowitz

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

Original languageEnglish (US)
Pages (from-to)2365-2376
Number of pages12
JournalNucleic Acids Research
Volume8
Issue number11
DOIs
StatePublished - Jun 11 1980
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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