Transcription of killer virion double-stranded RNA in vitro

J. Douglas Welsh, Michael J Leibowitz

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

Original languageEnglish (US)
Pages (from-to)2365-2376
Number of pages12
JournalNucleic Acids Research
Volume8
Issue number11
DOIs
StatePublished - Jun 11 1980
Externally publishedYes

Fingerprint

Double-Stranded RNA
Transcription
RNA
Virion
Homology
Sequence Homology
RNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Yeast
Stationary Phase
Saccharomyces cerevisiae
Ethanol
Saccharomyces Cerevisiae
Plasmids
Synthesis
In Vitro Techniques
Cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Transcription of killer virion double-stranded RNA in vitro. / Welsh, J. Douglas; Leibowitz, Michael J.

In: Nucleic Acids Research, Vol. 8, No. 11, 11.06.1980, p. 2365-2376.

Research output: Contribution to journalArticle

@article{80981563f6d9490cb274c9592c66ecea,
title = "Transcription of killer virion double-stranded RNA in vitro",
abstract = "Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.",
author = "Welsh, {J. Douglas} and Leibowitz, {Michael J}",
year = "1980",
month = "6",
day = "11",
doi = "10.1093/nar/8.11.2365",
language = "English (US)",
volume = "8",
pages = "2365--2376",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Transcription of killer virion double-stranded RNA in vitro

AU - Welsh, J. Douglas

AU - Leibowitz, Michael J

PY - 1980/6/11

Y1 - 1980/6/11

N2 - Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

AB - Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 × 106 dalton) and L (3.2 × 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.

UR - http://www.scopus.com/inward/record.url?scp=0019322275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019322275&partnerID=8YFLogxK

U2 - 10.1093/nar/8.11.2365

DO - 10.1093/nar/8.11.2365

M3 - Article

C2 - 7003534

AN - SCOPUS:0019322275

VL - 8

SP - 2365

EP - 2376

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 11

ER -