Trans-Fatty Acid-Stimulated Mammary Gland Growth in Ovariectomized Mice is Fatty Acid Type and Isomer Specific

Grace E. Berryhill, Susan G. Miszewski, Josephine F. Trott, Jana Kraft, Adam L. Lock, Russell C. Hovey

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We previously reported that the trans-18:2 fatty acid trans-10, cis-12 conjugated linoleic acid (t10,c12-CLA) stimulates mammary gland development independent of estrogen and its receptor. Given the negative consequences of dietary trans-fatty acids on various aspects of human health, we sought to establish whether other trans-fatty acids could similarly induce ovary-independent mammary gland growth in mice. Prepubertal BALB/cJ mice were ovariectomized at 21 days of age then were fed diets enriched with cis-9, trans-11 CLA (c9,t11-CLA), or mixtures of trans-18:1 fatty acids supplied by partially hydrogenated sunflower, safflower, or linseed oil. The resultant mammary phenotype was evaluated 3 weeks later and compared to the growth response elicited by t10,c12-CLA, or the defined control diet. Whereas partially hydrogenated safflower oil increased mammary gland weight, none of the partially hydrogenated vegetable oils promoted mammary ductal growth. Similarly, the c9,t11-CLA supplemented diet was without effect on mammary development. Taken together, our data emphasize a unique effect of t10,c12-CLA in stimulating estrogen-independent mammary gland growth manifest as increased mammary ductal area and elongation that was not recapitulated by c9,t11-CLA or the partially hydrogenated vegetable oil diets.

Original languageEnglish (US)
Pages (from-to)223-233
Number of pages11
Issue number3
StatePublished - Mar 1 2017


  • Conjugated linoleic acid
  • Mammary gland
  • Partially hydrogenated vegetable oil
  • Trans-fatty acids

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


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