Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection

Barbara L. Shacklett, Catherine A. Cox, Johan K. Sandberg, Neil H. Stollman, Mark A. Jacobson, Douglas F. Nixon

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Gut-associated lymphoid tissue (GALT) is a significant but understudied lymphoid organ, harboring a majority of the body's total lymphocyte population. GALT is also an important portal of entry for human immunodeficiency virus (HIV), a major site of viral replication and CD4+ T-cell depletion, and a frequent site of AIDS-related opportunistic infections and neoplasms. However, little is known about HIV-specific cell-mediated immune responses in GALT. Using lymphocytes isolated from rectal biopsies, we have determined the frequency and phenotype of HIV-specific CD8+ T cells in human GALT. GALT CD8+ T cells were predominantly CD45RO+ and expressed CXCR4 and CCR5. In 10 clinically stable, chronically infected individuals, the frequency of HIV Gag (SL9)-specific CD8+ T cells was increased in GALT relative to peripheral blood mononuclear cells by up to 4.6-fold, while that of cytomegalovirus (CMV)-specific CD8+ T cells was significantly reduced (P = 0.012). Both HIV- and CMV-specific CD8+ T cells in GALT expressed CCR5, but only HIV-specific CD8+ T cells expressed αEβ7 integrin, suggesting that mucosal priming may account for their retention in GALT. Chronically infected individuals exhibited striking depletion of GALT CD4+ T cells expressing CXCR4, CCR5, and αEβ7 integrin, but CD4+/CD8+ T-cell ratios in blood and GALT were similar. The percentage of GALT CD8+ T cells expressing αEβ7 was significantly decreased in infected individuals, suggesting that HIV infection may perturb lymphocyte retention in GALT. These studies demonstrate the feasibility of using tetramers to assess HIV-specific T cells in GALT and reveal that GALT is the site of an active CD8+ T-cell response during chronic infection.

Original languageEnglish (US)
Pages (from-to)5621-5631
Number of pages11
JournalJournal of Virology
Volume77
Issue number10
DOIs
StatePublished - May 2003
Externally publishedYes

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Lymphoid Tissue
Human immunodeficiency virus 1
HIV-1
digestive system
T-lymphocytes
T-Lymphocytes
Human immunodeficiency virus
Infection
infection
HIV
Cytomegalovirus
lymphocytes
integrins
Lymphocytes
tissues
Integrins
AIDS-Related Opportunistic Infections
HIV infections
Feasibility Studies
Virus Diseases

ASJC Scopus subject areas

  • Immunology

Cite this

Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection. / Shacklett, Barbara L.; Cox, Catherine A.; Sandberg, Johan K.; Stollman, Neil H.; Jacobson, Mark A.; Nixon, Douglas F.

In: Journal of Virology, Vol. 77, No. 10, 05.2003, p. 5621-5631.

Research output: Contribution to journalArticle

Shacklett, Barbara L. ; Cox, Catherine A. ; Sandberg, Johan K. ; Stollman, Neil H. ; Jacobson, Mark A. ; Nixon, Douglas F. / Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection. In: Journal of Virology. 2003 ; Vol. 77, No. 10. pp. 5621-5631.
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