Tracheobronchial epithelium in the adult rhesus monkey: a quantitative histochemical and ultrastructural study

Charles Plopper, J. G. Heidsiek, A. J. Weir, J. A. St. George, D. M. Hyde

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Abstract

Previous studies of the intrapulmonary conducting airways of sheep and rabbit have demonstrated marked diversity in the epithelial populations lining them. Because studies of trachea and centriacinar regions of macaque monkeys suggested that primates may be even more diverse, the present study was designed to characterize the epithelial population throughout the airway tree of one primate species, the rhesus monkey. Trachea and intrapulmonary airways of the right cranial and middle lobes of glutaraldehyde/paraformaldehyde-infused lungs of five adult rhesus monkeys were microdissected following the axial pathway. Each branch was assigned a binary number indicating its specific location within the tree. The trachea and six generations of intrapulmonary airway from the right cranial lobe were evaluated for ultrastructural and quantitative histology as were those of the right middle lobe for quantitative carbohydrate histochemistry. Four cell types were identified throughout the tree: ciliated, mucous goblet, small mucous granule, and basal. The tallest epithelium lined the trachea; the shortest, the respiratory bronchiole. The most cells per unit length of basement membrane were in proximal intrapulmonary bronchi; the least, in the respiratory bronchiole. The nonciliated bronchiolar epithelial or Clara cell was restricted to respiratory bronchioles. Sulfomucins were present in the vast majority of surface goblet cells in the trachea and proximal bronchi. In proximal bronchi, neutral glycoconjugates predominated in glands and acidic glycoconjugates in surface epithelium. In terminal and respiratory bronchioles the ratio of acidic glycoconjugate to neutral glycoconjugate equaled that in proximal bronchi, although glands were not present. Sulfomucins were minimal in terminal airways. We conclude that the characteristics of the epithelial lining of the mammalian tracheobronchial airway tree are very species-specific. The lining of the rhesus monkey does not have the diversity in cell types in different airway generations observed in sheep and rabbit. Also, the populations lining these airways in the rhesus are very different from either the sheep or rabbit in number, proportions of different cell types, glycoconjugate content, and distribution of specific cell types.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalAmerican Journal of Anatomy
Volume184
Issue number1
StatePublished - 1989

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Bronchioles
Macaca mulatta
Glycoconjugates
Trachea
Epithelium
Bronchi
Sheep
Rabbits
Primates
Population
Goblet Cells
Macaca
Glutaral
Basement Membrane
Haplorhini
Histology
Carbohydrates
Lung

ASJC Scopus subject areas

  • Anatomy

Cite this

Tracheobronchial epithelium in the adult rhesus monkey : a quantitative histochemical and ultrastructural study. / Plopper, Charles; Heidsiek, J. G.; Weir, A. J.; St. George, J. A.; Hyde, D. M.

In: American Journal of Anatomy, Vol. 184, No. 1, 1989, p. 31-40.

Research output: Contribution to journalArticle

Plopper, Charles ; Heidsiek, J. G. ; Weir, A. J. ; St. George, J. A. ; Hyde, D. M. / Tracheobronchial epithelium in the adult rhesus monkey : a quantitative histochemical and ultrastructural study. In: American Journal of Anatomy. 1989 ; Vol. 184, No. 1. pp. 31-40.
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