Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity

Emerson Soares Bernardes, Neide M. Silva, Luciana Pereira Ruas, Jose Roberta Mineo, Adriano Motta Loyola, Daniel K. Hsu, Fu-Tong Liu, Roger Chammas, Maria Cristina Roque-Barreira

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

In attempts to investigate the role of galectin-3 in innate immunity, we studied galectin-3-deficient (gal3-/-) mice with regard to their response to Toxoplasma gondii infection, which is characterized by inflammation in affected organs, Th-1-polarized immune response, and accumulation of cysts in the central nervous system. In wild-type (gal3+/+) mice, infected orally, galectin-3 was highly expressed in the leukocytes infiltrating the intestines, liver, lungs, and brain. Compared with gal3+/+, infected gal3-/- mice developed reduced inflammatory response in all of these organs but the lungs. Brain of gal3-/- mice displayed a significantly reduced number of infiltrating monocytes/macrophages and CD8+ cells and a higher parasite burden. Furthermore, gal3-/- mice mounted a higher Th1-polarized response and had comparable survival rates on peroral T. gondii infection, even though they were more susceptible to intraperitoneal infection. Interestingly, splenic cells and purified CD11c+ dendritic cells from gal3-/- mice produced higher amounts of interleukin-12 than cells from gal3+/+ mice, possibly explaining the higher Th1 response verified in the gal3-/- mice. We conclude that galectin-3 exerts an important role in innate immunity, including not only a proinflammatory effect but also a regulatory role on dendritic cells, capable of interfering in the adaptive immune response.

Original languageEnglish (US)
Pages (from-to)1910-1920
Number of pages11
JournalAmerican Journal of Pathology
Volume168
Issue number6
DOIs
StatePublished - Jun 2006

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Galectin 3
Toxoplasmosis
Adaptive Immunity
Innate Immunity
Dendritic Cells
Central Nervous System Cysts
Lung
Brain
Interleukin-12
Intestines
Monocytes
Parasites
Leukocytes
Macrophages
Inflammation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bernardes, E. S., Silva, N. M., Ruas, L. P., Mineo, J. R., Loyola, A. M., Hsu, D. K., ... Roque-Barreira, M. C. (2006). Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity. American Journal of Pathology, 168(6), 1910-1920. https://doi.org/10.2353/ajpath.2006.050636

Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity. / Bernardes, Emerson Soares; Silva, Neide M.; Ruas, Luciana Pereira; Mineo, Jose Roberta; Loyola, Adriano Motta; Hsu, Daniel K.; Liu, Fu-Tong; Chammas, Roger; Roque-Barreira, Maria Cristina.

In: American Journal of Pathology, Vol. 168, No. 6, 06.2006, p. 1910-1920.

Research output: Contribution to journalArticle

Bernardes, ES, Silva, NM, Ruas, LP, Mineo, JR, Loyola, AM, Hsu, DK, Liu, F-T, Chammas, R & Roque-Barreira, MC 2006, 'Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity', American Journal of Pathology, vol. 168, no. 6, pp. 1910-1920. https://doi.org/10.2353/ajpath.2006.050636
Bernardes, Emerson Soares ; Silva, Neide M. ; Ruas, Luciana Pereira ; Mineo, Jose Roberta ; Loyola, Adriano Motta ; Hsu, Daniel K. ; Liu, Fu-Tong ; Chammas, Roger ; Roque-Barreira, Maria Cristina. / Toxoplasma gondii infection reveals a novel regulatory role for galectin-3 in the interface of innate and adaptive immunity. In: American Journal of Pathology. 2006 ; Vol. 168, No. 6. pp. 1910-1920.
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