Toxoplasma Does Not Secrete the GRA16 and GRA24 Effectors Beyond the Parasitophorous Vacuole Membrane of Tissue Cysts

Shruthi Krishnamurthy; Jeroen Saeij

doi:10.3389/fcimb.2018.00366

Toxoplasma Does Not Secrete the GRA16 and GRA24 Effectors Beyond the Parasitophorous Vacuole Membrane of Tissue Cysts

Shruthi Krishnamurthy, Jeroen Saeij

Pathology, Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of Toxoplasma.

Original language	English (US)
Number of pages	1
Journal	Frontiers in cellular and infection microbiology
Volume	8
DOIs	https://doi.org/10.3389/fcimb.2018.00366
State	Published - Jan 1 2018

Keywords

GRA16
GRA24
secreted effectors
tetracycline inducible expression
tissue cyst wall
Toxoplasma gondii

ASJC Scopus subject areas

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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abstract = "After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of Toxoplasma.",

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