Abstract
After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of Toxoplasma.
| Original language | English (US) |
|---|---|
| Number of pages | 1 |
| Journal | Frontiers in cellular and infection microbiology |
| Volume | 8 |
| DOIs | |
| State | Published - Jan 1 2018 |
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Keywords
- GRA16
- GRA24
- secreted effectors
- tetracycline inducible expression
- tissue cyst wall
- Toxoplasma gondii
ASJC Scopus subject areas
- Microbiology
- Immunology
- Microbiology (medical)
- Infectious Diseases
Cite this
Toxoplasma Does Not Secrete the GRA16 and GRA24 Effectors Beyond the Parasitophorous Vacuole Membrane of Tissue Cysts. / Krishnamurthy, Shruthi; Saeij, Jeroen.
In: Frontiers in cellular and infection microbiology, Vol. 8, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxoplasma Does Not Secrete the GRA16 and GRA24 Effectors Beyond the Parasitophorous Vacuole Membrane of Tissue Cysts
AU - Krishnamurthy, Shruthi
AU - Saeij, Jeroen
PY - 2018/1/1
Y1 - 2018/1/1
N2 - After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of Toxoplasma.
AB - After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of Toxoplasma.
KW - GRA16
KW - GRA24
KW - secreted effectors
KW - tetracycline inducible expression
KW - tissue cyst wall
KW - Toxoplasma gondii
UR - http://www.scopus.com/inward/record.url?scp=85056259874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056259874&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2018.00366
DO - 10.3389/fcimb.2018.00366
M3 - Article
C2 - 30406043
AN - SCOPUS:85056259874
VL - 8
JO - Frontiers in cellular and infection microbiology
JF - Frontiers in cellular and infection microbiology
SN - 2235-2988
ER -