Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue

Jeroen Saeij, S. Coller, J. P. Boyle, M. E. Jerome, M. W. White, J. C. Boothroyd

Research output: Contribution to journalArticle

357 Citations (Scopus)

Abstract

Toxoplasma gondii, an obligate intracellular parasite of the phylum Apicomplexa, can cause severe disease in humans with an immature or suppressed immune system. The outcome of Toxoplasma infection is highly dependent on the strain type, as are many of its in vitro growth properties. Here we use genetic crosses between type II and III lines to show that strain-specific differences in the modulation of host cell transcription are mediated by a putative protein kinase, ROP16. Upon invasion by the parasite, this polymorphic protein is released from the apical organelles known as rhoptries and injected into the host cell, where it ultimately affects the activation of signal transducer and activator of transcription (STAT) signalling pathways and consequent downstream effects on a key host cytokine, interleukin (IL)-12. Our findings provide a new mechanism for how an intracellular eukaryotic pathogen can interact with its host and reveal important differences in how different Toxoplasma lineages have evolved to exploit this interaction.

Original languageEnglish (US)
Pages (from-to)324-327
Number of pages4
JournalNature
Volume445
Issue number7125
DOIs
StatePublished - Jan 18 2007
Externally publishedYes

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Toxoplasma
Phosphotransferases
Gene Expression
Injections
Parasites
Genetic Crosses
Apicomplexa
Interleukin-12
Transducers
Organelles
Protein Kinases
Immune System
Cytokines
Growth
Infection
Proteins

ASJC Scopus subject areas

  • General

Cite this

Saeij, J., Coller, S., Boyle, J. P., Jerome, M. E., White, M. W., & Boothroyd, J. C. (2007). Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. Nature, 445(7125), 324-327. https://doi.org/10.1038/nature05395

Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. / Saeij, Jeroen; Coller, S.; Boyle, J. P.; Jerome, M. E.; White, M. W.; Boothroyd, J. C.

In: Nature, Vol. 445, No. 7125, 18.01.2007, p. 324-327.

Research output: Contribution to journalArticle

Saeij, J, Coller, S, Boyle, JP, Jerome, ME, White, MW & Boothroyd, JC 2007, 'Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue', Nature, vol. 445, no. 7125, pp. 324-327. https://doi.org/10.1038/nature05395
Saeij J, Coller S, Boyle JP, Jerome ME, White MW, Boothroyd JC. Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. Nature. 2007 Jan 18;445(7125):324-327. https://doi.org/10.1038/nature05395
Saeij, Jeroen ; Coller, S. ; Boyle, J. P. ; Jerome, M. E. ; White, M. W. ; Boothroyd, J. C. / Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. In: Nature. 2007 ; Vol. 445, No. 7125. pp. 324-327.
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