Toxicity of chemical components of fine particles inhaled by aged rats: Effects of concentration

Michael T. Kleinman, Dallas M. Hyde, Charles Bufalino, Carol Basbaum, Deepak K. Bhalla, William J. Mautz

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


This study tested the hypothesis that exposure to mixtures containing fine particles and ozone (O3) would cause pulmonary injury and decrements in functions of immunological cells in exposed rats (22-24 months old) in a dose-dependent manner. Rats were exposed to high and low concentrations of ammonium bisulfate and elemental carbon and to 0.2 ppm O3. Control groups were exposed to purified air or O3 alone. The biological end points measured included histopathological markers of lung injury, bronchoalveolar lung fluid proteins, and, measures of the function of the lung's innate immunological defenses (macrophage antigen-directed phagocytosis and respiratory burst activity). Exposure to O3 alone at 0.2 ppm did not result in significant changes in any of the measured end points. Exposures to the particle mixtures plus O3 produced statistically significant changes consistent with adverse effects. The low-concentration mixture produced effects that were statistically significant compared to purified air but, with the exception of macrophage Fc receptor binding, exposure to the high-concentration mixture did not. The effects of the low- and high- concentration mixtures were not significantly different. The study supports previous work that indicated that particle + O3 mixtures were more toxic than O3 alone.

Original languageEnglish (US)
Pages (from-to)1080-1087
Number of pages8
JournalJournal of the Air and Waste Management Association
Issue number9
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Atmospheric Science
  • Waste Management and Disposal
  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry


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