Abstract
This study was designed to estimate the toxic threshold of male and female fish to microcystins based on different biomarkers. Japanese medaka (Oryzias latipes) were fed dietary Microcystin-LR (0, 0.46, 0.85, 2.01 and 3.93 μg MC-LR/g dry diet for 8 weeks at 25 °C. The results revealed that dietary MC-LR inhibited growth at the end of 8 weeks. The survival of embryos and the RNA/DNA ratio of whole fish decreased significantly (P < 0.05) in fish fed 3.93 μg MC-LR/g dry diet. Heat shock protein (Hsp60) expression was induced in the liver of female and male fish fed diets containing ≥0.85 and 0.46 μg MC-LR/g diet, respectively. The activity of liver caspase 3/7 was significantly higher in female fish fed 3.93 μg MC-LR/g diet and in males fed 2.01 MC-LR μg/g dry diet than fish fed the control diet. The threshold for inhibition of liver protein phosphatase expression was lower in female (2.01 μg/g diet) than that in male fish (3.93 μg/g diet). Histopathological examination showed significant single-cell necrosis in female and male medaka fed diets containing 0.85 and 3.93 μg MC-LR/g diet, respectively. Based on different biomarkers, this study demonstrated that dietary MC-LR is toxic to Medaka and the effects are gender dependent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 787-794 |
| Number of pages | 8 |
| Journal | Toxicon |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2010 |
Fingerprint
Keywords
- Apoptosis
- Medaka
- Microcystin
- Protein phosphatase
- Reproduction
- Stress protein
ASJC Scopus subject areas
- Toxicology
- Medicine(all)
Cite this
Toxic threshold of dietary microcystin (-LR) for quart medaka. / Deng, Dong Fang; Zheng, Keke; Teh, Foo Ching; Lehman, Peggy W.; Teh, Swee J.
In: Toxicon, Vol. 55, No. 4, 01.04.2010, p. 787-794.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxic threshold of dietary microcystin (-LR) for quart medaka
AU - Deng, Dong Fang
AU - Zheng, Keke
AU - Teh, Foo Ching
AU - Lehman, Peggy W.
AU - Teh, Swee J
PY - 2010/4/1
Y1 - 2010/4/1
N2 - This study was designed to estimate the toxic threshold of male and female fish to microcystins based on different biomarkers. Japanese medaka (Oryzias latipes) were fed dietary Microcystin-LR (0, 0.46, 0.85, 2.01 and 3.93 μg MC-LR/g dry diet for 8 weeks at 25 °C. The results revealed that dietary MC-LR inhibited growth at the end of 8 weeks. The survival of embryos and the RNA/DNA ratio of whole fish decreased significantly (P < 0.05) in fish fed 3.93 μg MC-LR/g dry diet. Heat shock protein (Hsp60) expression was induced in the liver of female and male fish fed diets containing ≥0.85 and 0.46 μg MC-LR/g diet, respectively. The activity of liver caspase 3/7 was significantly higher in female fish fed 3.93 μg MC-LR/g diet and in males fed 2.01 MC-LR μg/g dry diet than fish fed the control diet. The threshold for inhibition of liver protein phosphatase expression was lower in female (2.01 μg/g diet) than that in male fish (3.93 μg/g diet). Histopathological examination showed significant single-cell necrosis in female and male medaka fed diets containing 0.85 and 3.93 μg MC-LR/g diet, respectively. Based on different biomarkers, this study demonstrated that dietary MC-LR is toxic to Medaka and the effects are gender dependent.
AB - This study was designed to estimate the toxic threshold of male and female fish to microcystins based on different biomarkers. Japanese medaka (Oryzias latipes) were fed dietary Microcystin-LR (0, 0.46, 0.85, 2.01 and 3.93 μg MC-LR/g dry diet for 8 weeks at 25 °C. The results revealed that dietary MC-LR inhibited growth at the end of 8 weeks. The survival of embryos and the RNA/DNA ratio of whole fish decreased significantly (P < 0.05) in fish fed 3.93 μg MC-LR/g dry diet. Heat shock protein (Hsp60) expression was induced in the liver of female and male fish fed diets containing ≥0.85 and 0.46 μg MC-LR/g diet, respectively. The activity of liver caspase 3/7 was significantly higher in female fish fed 3.93 μg MC-LR/g diet and in males fed 2.01 MC-LR μg/g dry diet than fish fed the control diet. The threshold for inhibition of liver protein phosphatase expression was lower in female (2.01 μg/g diet) than that in male fish (3.93 μg/g diet). Histopathological examination showed significant single-cell necrosis in female and male medaka fed diets containing 0.85 and 3.93 μg MC-LR/g diet, respectively. Based on different biomarkers, this study demonstrated that dietary MC-LR is toxic to Medaka and the effects are gender dependent.
KW - Apoptosis
KW - Medaka
KW - Microcystin
KW - Protein phosphatase
KW - Reproduction
KW - Stress protein
UR - http://www.scopus.com/inward/record.url?scp=77953424622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953424622&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2009.11.012
DO - 10.1016/j.toxicon.2009.11.012
M3 - Article
C2 - 19941879
AN - SCOPUS:77953424622
VL - 55
SP - 787
EP - 794
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 4
ER -