Toxic effects of hexane derivatives on cultured rat Schwann cells

Michihiro Kamijima, Gen Sobue, Gaku Ichihara, Eiji Shibata, Yuichiro Ono, Hidetaka Kondo, Maria Beatriz G Villanueva, Takayuki Ito, Terunori Mitsuma, Yasuhiro Takeuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalToxicology
Volume108
Issue number1-2
DOIs
StatePublished - Apr 15 1996
Externally publishedYes

Fingerprint

Poisons
Schwann Cells
Hexanes
Rats
Cells
Derivatives
Thymidine
Methyl n-Butyl Ketone
DNA
Scintillation
Sciatic Nerve
Cytotoxicity
Autoradiography
Cell Nucleus
Spectrometry
Sprague Dawley Rats
Culture Media
Spectrum Analysis

Keywords

  • 2,5-Dimethylfuran
  • 2,5-Hexanedione
  • Cell culture
  • Mitosis
  • Schwann

ASJC Scopus subject areas

  • Toxicology

Cite this

Kamijima, M., Sobue, G., Ichihara, G., Shibata, E., Ono, Y., Kondo, H., ... Takeuchi, Y. (1996). Toxic effects of hexane derivatives on cultured rat Schwann cells. Toxicology, 108(1-2), 25-31. https://doi.org/10.1016/S0300-483X(95)03267-J

Toxic effects of hexane derivatives on cultured rat Schwann cells. / Kamijima, Michihiro; Sobue, Gen; Ichihara, Gaku; Shibata, Eiji; Ono, Yuichiro; Kondo, Hidetaka; Villanueva, Maria Beatriz G; Ito, Takayuki; Mitsuma, Terunori; Takeuchi, Yasuhiro.

In: Toxicology, Vol. 108, No. 1-2, 15.04.1996, p. 25-31.

Research output: Contribution to journalArticle

Kamijima, M, Sobue, G, Ichihara, G, Shibata, E, Ono, Y, Kondo, H, Villanueva, MBG, Ito, T, Mitsuma, T & Takeuchi, Y 1996, 'Toxic effects of hexane derivatives on cultured rat Schwann cells', Toxicology, vol. 108, no. 1-2, pp. 25-31. https://doi.org/10.1016/S0300-483X(95)03267-J
Kamijima M, Sobue G, Ichihara G, Shibata E, Ono Y, Kondo H et al. Toxic effects of hexane derivatives on cultured rat Schwann cells. Toxicology. 1996 Apr 15;108(1-2):25-31. https://doi.org/10.1016/S0300-483X(95)03267-J
Kamijima, Michihiro ; Sobue, Gen ; Ichihara, Gaku ; Shibata, Eiji ; Ono, Yuichiro ; Kondo, Hidetaka ; Villanueva, Maria Beatriz G ; Ito, Takayuki ; Mitsuma, Terunori ; Takeuchi, Yasuhiro. / Toxic effects of hexane derivatives on cultured rat Schwann cells. In: Toxicology. 1996 ; Vol. 108, No. 1-2. pp. 25-31.
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