Abstract
The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25-31 |
| Number of pages | 7 |
| Journal | Toxicology |
| Volume | 108 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Apr 15 1996 |
| Externally published | Yes |
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Keywords
- 2,5-Dimethylfuran
- 2,5-Hexanedione
- Cell culture
- Mitosis
- Schwann
ASJC Scopus subject areas
- Toxicology
Cite this
Toxic effects of hexane derivatives on cultured rat Schwann cells. / Kamijima, Michihiro; Sobue, Gen; Ichihara, Gaku; Shibata, Eiji; Ono, Yuichiro; Kondo, Hidetaka; Villanueva, Maria Beatriz G; Ito, Takayuki; Mitsuma, Terunori; Takeuchi, Yasuhiro.
In: Toxicology, Vol. 108, No. 1-2, 15.04.1996, p. 25-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxic effects of hexane derivatives on cultured rat Schwann cells
AU - Kamijima, Michihiro
AU - Sobue, Gen
AU - Ichihara, Gaku
AU - Shibata, Eiji
AU - Ono, Yuichiro
AU - Kondo, Hidetaka
AU - Villanueva, Maria Beatriz G
AU - Ito, Takayuki
AU - Mitsuma, Terunori
AU - Takeuchi, Yasuhiro
PY - 1996/4/15
Y1 - 1996/4/15
N2 - The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.
AB - The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.
KW - 2,5-Dimethylfuran
KW - 2,5-Hexanedione
KW - Cell culture
KW - Mitosis
KW - Schwann
UR - http://www.scopus.com/inward/record.url?scp=0029930572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029930572&partnerID=8YFLogxK
U2 - 10.1016/S0300-483X(95)03267-J
DO - 10.1016/S0300-483X(95)03267-J
M3 - Article
C2 - 8644114
AN - SCOPUS:0029930572
VL - 108
SP - 25
EP - 31
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 1-2
ER -