Toxic effects of hexane derivatives on cultured rat Schwann cells

Michihiro Kamijima, Gen Sobue, Gaku Ichihara, Eiji Shibata, Yuichiro Ono, Hidetaka Kondo, Maria Beatriz G Villanueva, Takayuki Ito, Terunori Mitsuma, Yasuhiro Takeuchi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalToxicology
Volume108
Issue number1-2
DOIs
StatePublished - Apr 15 1996
Externally publishedYes

Keywords

  • 2,5-Dimethylfuran
  • 2,5-Hexanedione
  • Cell culture
  • Mitosis
  • Schwann

ASJC Scopus subject areas

  • Toxicology

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  • Cite this

    Kamijima, M., Sobue, G., Ichihara, G., Shibata, E., Ono, Y., Kondo, H., Villanueva, M. B. G., Ito, T., Mitsuma, T., & Takeuchi, Y. (1996). Toxic effects of hexane derivatives on cultured rat Schwann cells. Toxicology, 108(1-2), 25-31. https://doi.org/10.1016/S0300-483X(95)03267-J