Abstract
The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF), and γ-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [3H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [3H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.
Original language | English (US) |
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Pages (from-to) | 25-31 |
Number of pages | 7 |
Journal | Toxicology |
Volume | 108 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 15 1996 |
Externally published | Yes |
Keywords
- 2,5-Dimethylfuran
- 2,5-Hexanedione
- Cell culture
- Mitosis
- Schwann
ASJC Scopus subject areas
- Toxicology