Toward the second generation of optogenetic tools

Thomas Knöpfel, Michael Z. Lin, Anselm Levskaya, Lin Tian, John Y. Lin, Edward S. Boyden

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


This mini-symposium aims to provide an integrated perspective on recent developments in optogenetics. Research in this emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.

Original languageEnglish (US)
Pages (from-to)14998-15004
Number of pages7
JournalJournal of Neuroscience
Issue number45
StatePublished - Nov 10 2010
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


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