Touchscreen learning deficits and normal social approach behavior in the Shank3B model of Phelan-McDermid Syndrome and autism

Nycole A. Copping, Elizabeth L. Berg, Gillian M. Foley, Melanie D. Schaffler, Beth L. Onaga, Nathalie Buscher, Jill L Silverman, Mu Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

SHANK3 is a synaptic scaffolding protein localized in the postsynaptic density and has a crucial role in synaptogenesis and neural physiology. Deletions and point mutations in SHANK3 cause Phelan-McDermid Syndrome (PMS), and have also been implicated in autism spectrum disorder (ASD) and intellectual disabilities, leading to the hypothesis that reduced SHANK3 expression impairs basic brain functions that are important for social communication and cognition. Several mouse models of Shank3 deletions have been generated, varying in the specific domain deleted. Here we report impairments in cognitive function in mice heterozygous for exon 13-16 (coding for the PDZ domain) deletion. The touchscreen pairwise discrimination task was chosen by virtue of its: (a) conceptual and technical similarities to the Cambridge Neuropsychological Test Automated Battery (CANTAB) and NIH Toolbox Cognition Battery used for testing cognitive functions in humans, (b) minimal demand on motor abilities, and (c) capability to measure many aspects of learning and memory and complex cognitive functions, including cognitive flexibility. The similarity between our mouse tasks and human cognitive assays means a high translational validity in future intervention studies using preclinical models. Our study revealed that Shank3B heterozygous mice (+/-) were slower to reach criterion in the pairwise visual discrimination task, and exhibited trends toward making more errors (first trial errors) and more correction errors than wildtype mice (+/+). Open field activity was normal in +/-, ruling out hypo- or hyperactivity as potential confounds in the touchscreen test. Sociability in the three chamber test was also normal in both +/+ and +/-These results indicate a deficit in discrimination learning in the Shank3B model of PMS and ASD, suggesting that this mouse model is a useful preclinical tool for studying neurobiological mechanisms behind cognitive impairments in PMS and ASD. The current findings are the starting point for our future research in which we will investigate multiple domains of cognition and explore pharmacological interventions.

Original languageEnglish (US)
JournalNeuroscience
DOIs
StateAccepted/In press - 2016

Fingerprint

Choice Behavior
Autistic Disorder
Cognition
Learning
PDZ Domains
Discrimination Learning
Post-Synaptic Density
Aptitude
Sequence Deletion
Neuropsychological Tests
Point Mutation
Intellectual Disability
Telomeric 22q13 Monosomy Syndrome
Exons
Communication
Pharmacology
Brain
Autism Spectrum Disorder

Keywords

  • Associative learning
  • Autism
  • Mouse models
  • Phelan-McDermid Syndrome
  • SHANK3
  • Touchscreen

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Copping, N. A., Berg, E. L., Foley, G. M., Schaffler, M. D., Onaga, B. L., Buscher, N., ... Yang, M. (Accepted/In press). Touchscreen learning deficits and normal social approach behavior in the Shank3B model of Phelan-McDermid Syndrome and autism. Neuroscience. https://doi.org/10.1016/j.neuroscience.2016.05.016

Touchscreen learning deficits and normal social approach behavior in the Shank3B model of Phelan-McDermid Syndrome and autism. / Copping, Nycole A.; Berg, Elizabeth L.; Foley, Gillian M.; Schaffler, Melanie D.; Onaga, Beth L.; Buscher, Nathalie; Silverman, Jill L; Yang, Mu.

In: Neuroscience, 2016.

Research output: Contribution to journalArticle

Copping, Nycole A. ; Berg, Elizabeth L. ; Foley, Gillian M. ; Schaffler, Melanie D. ; Onaga, Beth L. ; Buscher, Nathalie ; Silverman, Jill L ; Yang, Mu. / Touchscreen learning deficits and normal social approach behavior in the Shank3B model of Phelan-McDermid Syndrome and autism. In: Neuroscience. 2016.
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