Total Number and Mean Size of Alveoli in Mammalian Lung Estimated Using Fractionator Sampling and Unbiased Estimates of the Euler Characteristic of Alveolar Openings

Dallas M. Hyde, N. K. Tyler, L. F. Putney, P. Singh, H. J G Gundersen

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Estimation of alveolar number in the lung has traditionally been done by assuming a geometric shape and counting alveolar profiles in single, independent sections. In this study, we used the unbiased disector principle to estimate the Euler characteristic (and thereby the number) of alveolar openings in rat lungs and rhesus monkey lung lobes and to obtain robust estimates of average alveolar volume. The estimator of total alveolar number was based on systematic, uniformly random sampling using the fractionator sampling design. The number of alveoli in the rat lung ranged from 17.3 · 106 to 24.6 · 106, with a mean of 20.1 · 106. The average number of alveoli in the two left lung lobes in the monkey ranged from 48.8 · 106 to 67.1 · 106 with a mean of 57.7 · 106. The coefficient of error due to stereological sampling was of the order of 0.06 in both rats and monkeys and the biological variation (coefficient of variance between individuals) was 0.15 in rat and 0. 13 in monkey (left lobe, only). Between subdivisions (left/right in rat and cranial/caudal in monkey) there was an increase in variation, most markedly in the rat. With age (2 - 13 years) the alveolar volume increased 3-fold (as did parenchymal volume) in monkeys, but the alveolar number was unchanged. This study illustrates that use of the Euler characteristic and fractionator sampling is a robust and efficient, unbiased principle for the estimation of total alveolar number in the lung or in well-defined parts of it.

Original languageEnglish (US)
Pages (from-to)216-226
Number of pages11
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume277
Issue number1
StatePublished - Mar 2004

Fingerprint

lungs
Haplorhini
monkeys
Lung
rats
sampling
fold
Macaca mulatta
alveolus

Keywords

  • Alveoli
  • Biological variation
  • Disector
  • Euler characteristic
  • Fractionator
  • Monkey
  • Rat
  • Stereology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Total Number and Mean Size of Alveoli in Mammalian Lung Estimated Using Fractionator Sampling and Unbiased Estimates of the Euler Characteristic of Alveolar Openings. / Hyde, Dallas M.; Tyler, N. K.; Putney, L. F.; Singh, P.; Gundersen, H. J G.

In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 277, No. 1, 03.2004, p. 216-226.

Research output: Contribution to journalArticle

@article{08798d545d044335b5792cf84fa74d14,
title = "Total Number and Mean Size of Alveoli in Mammalian Lung Estimated Using Fractionator Sampling and Unbiased Estimates of the Euler Characteristic of Alveolar Openings",
abstract = "Estimation of alveolar number in the lung has traditionally been done by assuming a geometric shape and counting alveolar profiles in single, independent sections. In this study, we used the unbiased disector principle to estimate the Euler characteristic (and thereby the number) of alveolar openings in rat lungs and rhesus monkey lung lobes and to obtain robust estimates of average alveolar volume. The estimator of total alveolar number was based on systematic, uniformly random sampling using the fractionator sampling design. The number of alveoli in the rat lung ranged from 17.3 · 106 to 24.6 · 106, with a mean of 20.1 · 106. The average number of alveoli in the two left lung lobes in the monkey ranged from 48.8 · 106 to 67.1 · 106 with a mean of 57.7 · 106. The coefficient of error due to stereological sampling was of the order of 0.06 in both rats and monkeys and the biological variation (coefficient of variance between individuals) was 0.15 in rat and 0. 13 in monkey (left lobe, only). Between subdivisions (left/right in rat and cranial/caudal in monkey) there was an increase in variation, most markedly in the rat. With age (2 - 13 years) the alveolar volume increased 3-fold (as did parenchymal volume) in monkeys, but the alveolar number was unchanged. This study illustrates that use of the Euler characteristic and fractionator sampling is a robust and efficient, unbiased principle for the estimation of total alveolar number in the lung or in well-defined parts of it.",
keywords = "Alveoli, Biological variation, Disector, Euler characteristic, Fractionator, Monkey, Rat, Stereology",
author = "Hyde, {Dallas M.} and Tyler, {N. K.} and Putney, {L. F.} and P. Singh and Gundersen, {H. J G}",
year = "2004",
month = "3",
language = "English (US)",
volume = "277",
pages = "216--226",
journal = "Anatomical Record",
issn = "1932-8486",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Total Number and Mean Size of Alveoli in Mammalian Lung Estimated Using Fractionator Sampling and Unbiased Estimates of the Euler Characteristic of Alveolar Openings

AU - Hyde, Dallas M.

AU - Tyler, N. K.

AU - Putney, L. F.

AU - Singh, P.

AU - Gundersen, H. J G

PY - 2004/3

Y1 - 2004/3

N2 - Estimation of alveolar number in the lung has traditionally been done by assuming a geometric shape and counting alveolar profiles in single, independent sections. In this study, we used the unbiased disector principle to estimate the Euler characteristic (and thereby the number) of alveolar openings in rat lungs and rhesus monkey lung lobes and to obtain robust estimates of average alveolar volume. The estimator of total alveolar number was based on systematic, uniformly random sampling using the fractionator sampling design. The number of alveoli in the rat lung ranged from 17.3 · 106 to 24.6 · 106, with a mean of 20.1 · 106. The average number of alveoli in the two left lung lobes in the monkey ranged from 48.8 · 106 to 67.1 · 106 with a mean of 57.7 · 106. The coefficient of error due to stereological sampling was of the order of 0.06 in both rats and monkeys and the biological variation (coefficient of variance between individuals) was 0.15 in rat and 0. 13 in monkey (left lobe, only). Between subdivisions (left/right in rat and cranial/caudal in monkey) there was an increase in variation, most markedly in the rat. With age (2 - 13 years) the alveolar volume increased 3-fold (as did parenchymal volume) in monkeys, but the alveolar number was unchanged. This study illustrates that use of the Euler characteristic and fractionator sampling is a robust and efficient, unbiased principle for the estimation of total alveolar number in the lung or in well-defined parts of it.

AB - Estimation of alveolar number in the lung has traditionally been done by assuming a geometric shape and counting alveolar profiles in single, independent sections. In this study, we used the unbiased disector principle to estimate the Euler characteristic (and thereby the number) of alveolar openings in rat lungs and rhesus monkey lung lobes and to obtain robust estimates of average alveolar volume. The estimator of total alveolar number was based on systematic, uniformly random sampling using the fractionator sampling design. The number of alveoli in the rat lung ranged from 17.3 · 106 to 24.6 · 106, with a mean of 20.1 · 106. The average number of alveoli in the two left lung lobes in the monkey ranged from 48.8 · 106 to 67.1 · 106 with a mean of 57.7 · 106. The coefficient of error due to stereological sampling was of the order of 0.06 in both rats and monkeys and the biological variation (coefficient of variance between individuals) was 0.15 in rat and 0. 13 in monkey (left lobe, only). Between subdivisions (left/right in rat and cranial/caudal in monkey) there was an increase in variation, most markedly in the rat. With age (2 - 13 years) the alveolar volume increased 3-fold (as did parenchymal volume) in monkeys, but the alveolar number was unchanged. This study illustrates that use of the Euler characteristic and fractionator sampling is a robust and efficient, unbiased principle for the estimation of total alveolar number in the lung or in well-defined parts of it.

KW - Alveoli

KW - Biological variation

KW - Disector

KW - Euler characteristic

KW - Fractionator

KW - Monkey

KW - Rat

KW - Stereology

UR - http://www.scopus.com/inward/record.url?scp=1442335831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1442335831&partnerID=8YFLogxK

M3 - Article

C2 - 14983516

AN - SCOPUS:1442335831

VL - 277

SP - 216

EP - 226

JO - Anatomical Record

JF - Anatomical Record

SN - 1932-8486

IS - 1

ER -