Abstract
Heterotrimeric kinesin-2 motors [1,2] transport intraflagellar transport (IFT)-particles from the base to the tip of the axoneme to assemble and maintain cilia [3-10]. These motors are distinct in containing two non-identical motor subunits together with an accessory subunit [1,11-15]. We evaluated the significance of this organization by comparing purified wild type kinesin-2 holoenzymes that support IFT in vivo, with mutant trimers containing only one type of motor domain that do not support IFT in vivo. In motility assays, wild type kinesin-2 moved microtubules (MTs) at a rate intermediate between the rates supported by the two mutants. Interestingly, one of the mutants, but not the other mutant or the wild type protein, was observed to drive a persistent counter-clock-wise rotation of the gliding MTs. Thus one of the two motor domains of heterotrimeric kinesin-2 exerts torque as well as axial force as it moves along a MT, which may allow kinesin-2 to control its circumferential position around a MT doublet within the cilium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 53-57 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 401 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 8 2010 |
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Keywords
- Heterotrimeric kinesin-2
- Intraflagellar transport
- Torque generation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Torque generation by one of the motor subunits of heterotrimeric kinesin-2. / Pan, Xiaoyu; Acar, Seyda; Scholey, Jonathan M.
In: Biochemical and Biophysical Research Communications, Vol. 401, No. 1, 08.10.2010, p. 53-57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Torque generation by one of the motor subunits of heterotrimeric kinesin-2
AU - Pan, Xiaoyu
AU - Acar, Seyda
AU - Scholey, Jonathan M.
PY - 2010/10/8
Y1 - 2010/10/8
N2 - Heterotrimeric kinesin-2 motors [1,2] transport intraflagellar transport (IFT)-particles from the base to the tip of the axoneme to assemble and maintain cilia [3-10]. These motors are distinct in containing two non-identical motor subunits together with an accessory subunit [1,11-15]. We evaluated the significance of this organization by comparing purified wild type kinesin-2 holoenzymes that support IFT in vivo, with mutant trimers containing only one type of motor domain that do not support IFT in vivo. In motility assays, wild type kinesin-2 moved microtubules (MTs) at a rate intermediate between the rates supported by the two mutants. Interestingly, one of the mutants, but not the other mutant or the wild type protein, was observed to drive a persistent counter-clock-wise rotation of the gliding MTs. Thus one of the two motor domains of heterotrimeric kinesin-2 exerts torque as well as axial force as it moves along a MT, which may allow kinesin-2 to control its circumferential position around a MT doublet within the cilium.
AB - Heterotrimeric kinesin-2 motors [1,2] transport intraflagellar transport (IFT)-particles from the base to the tip of the axoneme to assemble and maintain cilia [3-10]. These motors are distinct in containing two non-identical motor subunits together with an accessory subunit [1,11-15]. We evaluated the significance of this organization by comparing purified wild type kinesin-2 holoenzymes that support IFT in vivo, with mutant trimers containing only one type of motor domain that do not support IFT in vivo. In motility assays, wild type kinesin-2 moved microtubules (MTs) at a rate intermediate between the rates supported by the two mutants. Interestingly, one of the mutants, but not the other mutant or the wild type protein, was observed to drive a persistent counter-clock-wise rotation of the gliding MTs. Thus one of the two motor domains of heterotrimeric kinesin-2 exerts torque as well as axial force as it moves along a MT, which may allow kinesin-2 to control its circumferential position around a MT doublet within the cilium.
KW - Heterotrimeric kinesin-2
KW - Intraflagellar transport
KW - Torque generation
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UR - http://www.scopus.com/inward/citedby.url?scp=77957667137&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.09.007
DO - 10.1016/j.bbrc.2010.09.007
M3 - Article
C2 - 20833139
AN - SCOPUS:77957667137
VL - 401
SP - 53
EP - 57
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -