Tor signaling and nutrient-based signals converge on Mks1p phosphorylation to regulate expression of Rtg1p·Rtg3p-dependent target genes

Ivanka Dilova, Sofia Aronova, Jenny C Y Chen, Ted Powers

Research output: Contribution to journalArticle

65 Scopus citations


The heterodimeric bZip/HLH transcription factors Rtg1p and Rtg3p regulate the expression of a concise set of metabolic genes (termed RTG target genes) required for de novo biosynthesis of glutamate and glutamine. Several components have now been identified that control both the intracellular localization as well as activity of the Rtg1p·Rtg3p complex, yet the precise upstream regulatory signals involved remain unclear. For example, it has been proposed that Btg1p·Rtg3p activity is repressed by glutamate, acting through the mitochondrial retrograde response pathway or, alternatively, by glutamine, acting through the Tor kinase pathway. Here we demonstrate that RTG target gene regulation is remarkably complex, with glutamate and glutamine as well as ammonia collaborating as potentially distinct signals to regulate RTG target gene expression. We show that both Tor and these nutrient-based signals converge on Mks1p, the immediate upstream inhibitor of Rtg1p·Rtg3p, and that a direct correlation exists between the degree of Mks1p phosphorylation and the extent of RTG target gene repression. Finally, we find that Tor- and glutamine-mediated RTG-target gene repression can be experimentally uncoupled, indicating that glutamine and Tor act, at least in part, independently to inhibit this pathway.

Original languageEnglish (US)
Pages (from-to)46527-46535
Number of pages9
JournalJournal of Biological Chemistry
Issue number45
StatePublished - Nov 5 2004


ASJC Scopus subject areas

  • Biochemistry

Cite this