TOR Complex 1 Includes a Novel Component, Tco89p (YPL180w), and Cooperates with Ssd1p to Maintain Cellular Integrity in Saccharomyces cerevisiae

Aaron Reinke, Scott Anderson, J. Michael McCaffery, John Yates, Sofia Aronova, Stephanie Chu, Stephen Fairclough, Cory Iverson, Karen P. Wedaman, Ted Powers

Research output: Contribution to journalArticle

164 Scopus citations

Abstract

The Tor1p and Tor2p kinases, targets of the therapeutically important antibiotic rapamycin, function as components of two distinct protein complexes in yeast, termed TOR complex 1 (TORC1) and TORC2. TORC1 is responsible for a wide range of rapamycin-sensitive cellular activities and contains, in addition to Tor1p or Tor2p, two highly conserved proteins, Lst8p and Kog1p. By identifying proteins that co-purify with Tor1p, Tor2p, Lst8p, and Kog1p, we have characterized a comprehensive set of protein-protein interactions that define further the composition of TORC1 as well as TORC2. In particular, we have identified Tco89p (YPL180w) and Bit61p (YJL058c) as novel components of TORC1 and TORC2, respectively. Deletion of TOR1 or TC089 results in two specific and distinct phenotypes, (i) rapamycin-hypersensitivity and (ii) decreased cellular integrity, both of which correlate with the presence of SSD1-d, an allele of SSD1 previously associated with defects in cellular integrity. Furthermore, we link Ssd1p to Tap42p, a component of the TOR pathway that is believed to act uniquely downstream of TORC1. Together, these results define a novel connection between TORC1 and Ssd1p-mediated maintenance of cellular integrity.

Original languageEnglish (US)
Pages (from-to)14752-14762
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number15
DOIs
StatePublished - Apr 9 2004

ASJC Scopus subject areas

  • Biochemistry

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    Reinke, A., Anderson, S., McCaffery, J. M., Yates, J., Aronova, S., Chu, S., Fairclough, S., Iverson, C., Wedaman, K. P., & Powers, T. (2004). TOR Complex 1 Includes a Novel Component, Tco89p (YPL180w), and Cooperates with Ssd1p to Maintain Cellular Integrity in Saccharomyces cerevisiae. Journal of Biological Chemistry, 279(15), 14752-14762. https://doi.org/10.1074/jbc.M313062200