Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo

Masaru Nakamoto, Hwai-Jong Cheng, Glenn C. Friedman, Todd McLaughlin, Michael J. Hansen, Cliff H. Yoon, Dennis D M O'Leary, John G. Flanagan

Research output: Contribution to journalArticle

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Abstract

Topographic maps, which maintain the spatial order of neurons in the order of their axonal connections, are found throughout the nervous system. In the visual retinotectal projection, ELF-1, a ligand in the tectum, and its receptors in the retina show complementary gradients in expression and binding, indicating they may be positional labels for map development. Here we show that ELF-1 acts as a repellent axon guidance factor in vitro. In vivo, when the tectal ELF-1 pattern is modified by retroviral overexpression, retinal axons avoid ectopic ELF-1 patches and map to abnormally anterior positions. All these effects were seen on axons from temporal but not nasal retina, indicating that ELF-1 could determine nasal versus temporal retinotectal specificity, and providing a direct demonstration of a cell recognition molecule with topographically specific effects on neural map development.

Original languageEnglish (US)
Pages (from-to)755-766
Number of pages12
JournalCell
Volume86
Issue number5
DOIs
StatePublished - Sep 6 1996
Externally publishedYes

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In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Nakamoto, M., Cheng, H-J., Friedman, G. C., McLaughlin, T., Hansen, M. J., Yoon, C. H., ... Flanagan, J. G. (1996). Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo. Cell, 86(5), 755-766. https://doi.org/10.1016/S0092-8674(00)80150-6

Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo. / Nakamoto, Masaru; Cheng, Hwai-Jong; Friedman, Glenn C.; McLaughlin, Todd; Hansen, Michael J.; Yoon, Cliff H.; O'Leary, Dennis D M; Flanagan, John G.

In: Cell, Vol. 86, No. 5, 06.09.1996, p. 755-766.

Research output: Contribution to journalArticle

Nakamoto, M, Cheng, H-J, Friedman, GC, McLaughlin, T, Hansen, MJ, Yoon, CH, O'Leary, DDM & Flanagan, JG 1996, 'Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo', Cell, vol. 86, no. 5, pp. 755-766. https://doi.org/10.1016/S0092-8674(00)80150-6
Nakamoto M, Cheng H-J, Friedman GC, McLaughlin T, Hansen MJ, Yoon CH et al. Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo. Cell. 1996 Sep 6;86(5):755-766. https://doi.org/10.1016/S0092-8674(00)80150-6
Nakamoto, Masaru ; Cheng, Hwai-Jong ; Friedman, Glenn C. ; McLaughlin, Todd ; Hansen, Michael J. ; Yoon, Cliff H. ; O'Leary, Dennis D M ; Flanagan, John G. / Topographically specific effects of ELF-1 on retinal axon guidance in vitro and retinal axon mapping in vivo. In: Cell. 1996 ; Vol. 86, No. 5. pp. 755-766.
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