Topographic requirements for signaling neutrophil activation through Lselectin

Scott Simon, David Pearson

Research output: Contribution to journalArticlepeer-review

Abstract

The recognition events and receptor rearrangements that lead to signaling through Lselectin were studied using antibodies and sialyl lewis-x (sLex) carbohydrate ligands. These were bound to neutrophils in solution or following attachment via their Fc domains to protein-A microspheres, and then activation was quantitated as upregulation of surface CD11b/CD18 (Mac-1) using fluorescence flow cytometry. In general, relatively high affinity binding and receptor clustering appeared to be a requirement for signal transduction through L-selectin.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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