Topical gentamicin-induced hearing loss: A mitochondrial ribosomal RNA study of genetic susceptibility

Joseph M. Chen, Peter A. Williamson, Tim Hutchin, Julian M. Nedzelski, Gino A Cortopassi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.

Original languageEnglish (US)
Pages (from-to)850-852
Number of pages3
JournalAmerican Journal of Otology
Volume17
Issue number6
StatePublished - Nov 1996

Fingerprint

Ribosomal RNA
Aminoglycosides
Genetic Predisposition to Disease
Gentamicins
Hearing Loss
Middle Ear
rRNA Genes
Mutation
Gene Amplification
DNA
Vertigo
Otitis Media
Pedigree
Hypersensitivity
Nucleotides
Anti-Bacterial Agents
Polymerase Chain Reaction
mitochondrial RNA
Therapeutics

Keywords

  • Gentamycin
  • Hearing loss
  • RNA mutation

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Topical gentamicin-induced hearing loss : A mitochondrial ribosomal RNA study of genetic susceptibility. / Chen, Joseph M.; Williamson, Peter A.; Hutchin, Tim; Nedzelski, Julian M.; Cortopassi, Gino A.

In: American Journal of Otology, Vol. 17, No. 6, 11.1996, p. 850-852.

Research output: Contribution to journalArticle

Chen, Joseph M. ; Williamson, Peter A. ; Hutchin, Tim ; Nedzelski, Julian M. ; Cortopassi, Gino A. / Topical gentamicin-induced hearing loss : A mitochondrial ribosomal RNA study of genetic susceptibility. In: American Journal of Otology. 1996 ; Vol. 17, No. 6. pp. 850-852.
@article{b0b0c58d8c9b4288ba8f4cfbc4fc3c06,
title = "Topical gentamicin-induced hearing loss: A mitochondrial ribosomal RNA study of genetic susceptibility",
abstract = "Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.",
keywords = "Gentamycin, Hearing loss, RNA mutation",
author = "Chen, {Joseph M.} and Williamson, {Peter A.} and Tim Hutchin and Nedzelski, {Julian M.} and Cortopassi, {Gino A}",
year = "1996",
month = "11",
language = "English (US)",
volume = "17",
pages = "850--852",
journal = "Otology and Neurotology",
issn = "1531-7129",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Topical gentamicin-induced hearing loss

T2 - A mitochondrial ribosomal RNA study of genetic susceptibility

AU - Chen, Joseph M.

AU - Williamson, Peter A.

AU - Hutchin, Tim

AU - Nedzelski, Julian M.

AU - Cortopassi, Gino A

PY - 1996/11

Y1 - 1996/11

N2 - Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.

AB - Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.

KW - Gentamycin

KW - Hearing loss

KW - RNA mutation

UR - http://www.scopus.com/inward/record.url?scp=0029974154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029974154&partnerID=8YFLogxK

M3 - Article

C2 - 8915412

AN - SCOPUS:0029974154

VL - 17

SP - 850

EP - 852

JO - Otology and Neurotology

JF - Otology and Neurotology

SN - 1531-7129

IS - 6

ER -