Topical gentamicin-induced hearing loss: A mitochondrial ribosomal RNA study of genetic susceptibility

Joseph M. Chen, Peter A. Williamson, Tim Hutchin, Julian M. Nedzelski, Gino A Cortopassi

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Mitochondrial ribosomal RNA mutation has been shown to predispose affected individuals to aminoglycoside-induced hearing loss (AIHL). An A-to-G nucleotide substitution at the 1555 position within the 12S ribosomal RNA gene has been identified with a maternally inherited pattern in affected Asian pedigrees. The aim of this study was to identify the frequency of this DNA polymorphism in subjects who appear to show hypersensitivity to topical (middle ear) application of aminoglycoside. In this pilot study, 10 subjects with AIHL were recruited. Eight underwent vestibular ablative therapy with middle ear instillation of gentamicin for disabling vertigo, and two were treated with topical antibiotic drops for otitis media in the presence of a tympanic perforation. DNA samples were extracted, and polymerase chain reaction (PCR) technique was used for gene amplification and purification, searching for 1555 A-to-G substitution in the 12S ribosomal RNA gene. None of the subjects demonstrated this specific mutation.

Original languageEnglish (US)
Pages (from-to)850-852
Number of pages3
JournalAmerican Journal of Otology
Volume17
Issue number6
StatePublished - Nov 1996

Keywords

  • Gentamycin
  • Hearing loss
  • RNA mutation

ASJC Scopus subject areas

  • Otorhinolaryngology

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