Topical application of yeast extract accelerates the wound healing of diabetic mice

Maria J. Crowe, Redmond B. McNeill, Donna J. Schlemm, David G Greenhalgh, Stephen J. Keller

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Alcoholic extracts of yeast have been used as the active ingredient in medications under names such as 'tissue or skin respiratory factor,' Biodyne (Sperti Drug Co, Cincinnati, Ohio - now defunct), and live yeast cell derivative (LYCD). Beneficial clinical results from the use of LYCD have been reported for the treatment of burns, wounds, and hemorrhoids. The medicinal effects of LYCD have recently been localized to a protein fraction containing a mixture of several peptides. The effects of topical application of the peptide mixture on wounds were examined in diabetic mice, an animal model in which the healing process is disrupted and delayed. Full-thickness wounds were created on the backs of diabetic (DB) and nondiabetic (non-DB) mice. Half of the DB and non-DB mice were treated with 0.05 mL of LYCD after wounding and for 4 successive days. All other mice received vehicle. Wound areas were measured at Day 0 and at 2-day intervals. Mice were sacrificed at 3, 7, 10, 21, and 28 days postinjury. Differences in the extent and quality of healing appeared between DB mice receiving LYCD and DB mice receiving vehicle by day 10 (P < .0001). By 24 days postinjury, DB mice receiving LYCD had achieved 100%wound closure, whereas DB mice receiving vehicle had achieved only 31.4% wound closure. Histologic examination of wounds reflected improved wound healing in DB mice receiving LYCD as compared with those receiving vehicle. A topically applied yeast extract peptide mixture significantly attenuates wound closure and the degree of cellular reorganization of full-thickness excisional wounds of DB mice.

Original languageEnglish (US)
Pages (from-to)155-162
Number of pages8
JournalJournal of Burn Care and Rehabilitation
Volume20
Issue number2
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Wound Healing
Yeasts
Wounds and Injuries
Peptides
Hemorrhoids
live yeast cell derivative
Burns
Names
Animal Models
Skin

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Topical application of yeast extract accelerates the wound healing of diabetic mice. / Crowe, Maria J.; McNeill, Redmond B.; Schlemm, Donna J.; Greenhalgh, David G; Keller, Stephen J.

In: Journal of Burn Care and Rehabilitation, Vol. 20, No. 2, 03.1999, p. 155-162.

Research output: Contribution to journalArticle

Crowe, Maria J. ; McNeill, Redmond B. ; Schlemm, Donna J. ; Greenhalgh, David G ; Keller, Stephen J. / Topical application of yeast extract accelerates the wound healing of diabetic mice. In: Journal of Burn Care and Rehabilitation. 1999 ; Vol. 20, No. 2. pp. 155-162.
@article{fbb21ce124d645ff853304ef4e62a4b1,
title = "Topical application of yeast extract accelerates the wound healing of diabetic mice",
abstract = "Alcoholic extracts of yeast have been used as the active ingredient in medications under names such as 'tissue or skin respiratory factor,' Biodyne (Sperti Drug Co, Cincinnati, Ohio - now defunct), and live yeast cell derivative (LYCD). Beneficial clinical results from the use of LYCD have been reported for the treatment of burns, wounds, and hemorrhoids. The medicinal effects of LYCD have recently been localized to a protein fraction containing a mixture of several peptides. The effects of topical application of the peptide mixture on wounds were examined in diabetic mice, an animal model in which the healing process is disrupted and delayed. Full-thickness wounds were created on the backs of diabetic (DB) and nondiabetic (non-DB) mice. Half of the DB and non-DB mice were treated with 0.05 mL of LYCD after wounding and for 4 successive days. All other mice received vehicle. Wound areas were measured at Day 0 and at 2-day intervals. Mice were sacrificed at 3, 7, 10, 21, and 28 days postinjury. Differences in the extent and quality of healing appeared between DB mice receiving LYCD and DB mice receiving vehicle by day 10 (P < .0001). By 24 days postinjury, DB mice receiving LYCD had achieved 100{\%}wound closure, whereas DB mice receiving vehicle had achieved only 31.4{\%} wound closure. Histologic examination of wounds reflected improved wound healing in DB mice receiving LYCD as compared with those receiving vehicle. A topically applied yeast extract peptide mixture significantly attenuates wound closure and the degree of cellular reorganization of full-thickness excisional wounds of DB mice.",
author = "Crowe, {Maria J.} and McNeill, {Redmond B.} and Schlemm, {Donna J.} and Greenhalgh, {David G} and Keller, {Stephen J.}",
year = "1999",
month = "3",
language = "English (US)",
volume = "20",
pages = "155--162",
journal = "Journal of Burn Care and Research",
issn = "1559-047X",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Topical application of yeast extract accelerates the wound healing of diabetic mice

AU - Crowe, Maria J.

AU - McNeill, Redmond B.

AU - Schlemm, Donna J.

AU - Greenhalgh, David G

AU - Keller, Stephen J.

PY - 1999/3

Y1 - 1999/3

N2 - Alcoholic extracts of yeast have been used as the active ingredient in medications under names such as 'tissue or skin respiratory factor,' Biodyne (Sperti Drug Co, Cincinnati, Ohio - now defunct), and live yeast cell derivative (LYCD). Beneficial clinical results from the use of LYCD have been reported for the treatment of burns, wounds, and hemorrhoids. The medicinal effects of LYCD have recently been localized to a protein fraction containing a mixture of several peptides. The effects of topical application of the peptide mixture on wounds were examined in diabetic mice, an animal model in which the healing process is disrupted and delayed. Full-thickness wounds were created on the backs of diabetic (DB) and nondiabetic (non-DB) mice. Half of the DB and non-DB mice were treated with 0.05 mL of LYCD after wounding and for 4 successive days. All other mice received vehicle. Wound areas were measured at Day 0 and at 2-day intervals. Mice were sacrificed at 3, 7, 10, 21, and 28 days postinjury. Differences in the extent and quality of healing appeared between DB mice receiving LYCD and DB mice receiving vehicle by day 10 (P < .0001). By 24 days postinjury, DB mice receiving LYCD had achieved 100%wound closure, whereas DB mice receiving vehicle had achieved only 31.4% wound closure. Histologic examination of wounds reflected improved wound healing in DB mice receiving LYCD as compared with those receiving vehicle. A topically applied yeast extract peptide mixture significantly attenuates wound closure and the degree of cellular reorganization of full-thickness excisional wounds of DB mice.

AB - Alcoholic extracts of yeast have been used as the active ingredient in medications under names such as 'tissue or skin respiratory factor,' Biodyne (Sperti Drug Co, Cincinnati, Ohio - now defunct), and live yeast cell derivative (LYCD). Beneficial clinical results from the use of LYCD have been reported for the treatment of burns, wounds, and hemorrhoids. The medicinal effects of LYCD have recently been localized to a protein fraction containing a mixture of several peptides. The effects of topical application of the peptide mixture on wounds were examined in diabetic mice, an animal model in which the healing process is disrupted and delayed. Full-thickness wounds were created on the backs of diabetic (DB) and nondiabetic (non-DB) mice. Half of the DB and non-DB mice were treated with 0.05 mL of LYCD after wounding and for 4 successive days. All other mice received vehicle. Wound areas were measured at Day 0 and at 2-day intervals. Mice were sacrificed at 3, 7, 10, 21, and 28 days postinjury. Differences in the extent and quality of healing appeared between DB mice receiving LYCD and DB mice receiving vehicle by day 10 (P < .0001). By 24 days postinjury, DB mice receiving LYCD had achieved 100%wound closure, whereas DB mice receiving vehicle had achieved only 31.4% wound closure. Histologic examination of wounds reflected improved wound healing in DB mice receiving LYCD as compared with those receiving vehicle. A topically applied yeast extract peptide mixture significantly attenuates wound closure and the degree of cellular reorganization of full-thickness excisional wounds of DB mice.

UR - http://www.scopus.com/inward/record.url?scp=0033065991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033065991&partnerID=8YFLogxK

M3 - Article

C2 - 10188114

AN - SCOPUS:0033065991

VL - 20

SP - 155

EP - 162

JO - Journal of Burn Care and Research

JF - Journal of Burn Care and Research

SN - 1559-047X

IS - 2

ER -