Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults

Christopher G L Hobbs, Eleanor Groves, Victoria Davenport, Michael Bailey, Neil A. Williams, Martin A. Birchall, Robert S. Heyderman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: Human papillomavirus (HPV) is known to infect the epithelium of the upper aerodigestive tract; however, major questions regarding prevalence and persistence of infection, and their relation to local immune response, remain unanswered. OBJECTIVES: To evaluate the tonsil T cell immune response to HPV and compare this to the frequency of detectable virus at this site. STUDY DESIGN: A cross-sectional study of cancer-free adults undergoing routine tonsillectomy. METHODS: Mucosal immune responses to recombinant HPV16 L2E6E7 and HPV6 L2E7 antigens were measured by tonsillar T-lymphocyte proliferation assay in 13 subjects. HPV deoxyribonucleic acid (DNA) was assessed by PCR and reverse line-blot hybridization in an expanded population of 44 subjects. RESULTS: Proliferative T-cell responses to HPV16 and HPV6 were identified in all patients. The presence of a CD45RO+ T cell population responsive to HPV6 L2E7 was confirmed in three of six subjects tested. There were no CD45RO+ responses to HPV16 L2E6E7 and no evidence of current or latent HPV infection of the tonsil. CONCLUSIONS: T cell memory to human papillomavirus can be identified in tonsil tissue from an adult population in the absence of concurrent HPV infection. How novel HPV vaccines might augment this preexisting cell-mediated immunity is an essential area for investigation.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalLaryngoscope
Volume118
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Palatine Tonsil
Immunity
Viruses
T-Lymphocytes
Papillomavirus Infections
Population
Papillomavirus Vaccines
Mucosal Immunity
Tonsillectomy
Cellular Immunity
Epithelium
Cross-Sectional Studies
Antigens
Polymerase Chain Reaction
DNA
Infection
Neoplasms

Keywords

  • Human papillomavirus
  • T cell immunity
  • Tonsil

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Hobbs, C. G. L., Groves, E., Davenport, V., Bailey, M., Williams, N. A., Birchall, M. A., & Heyderman, R. S. (2008). Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults. Laryngoscope, 118(3), 459-463. https://doi.org/10.1097/MLG.0b013e31815aedb3

Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults. / Hobbs, Christopher G L; Groves, Eleanor; Davenport, Victoria; Bailey, Michael; Williams, Neil A.; Birchall, Martin A.; Heyderman, Robert S.

In: Laryngoscope, Vol. 118, No. 3, 03.2008, p. 459-463.

Research output: Contribution to journalArticle

Hobbs, CGL, Groves, E, Davenport, V, Bailey, M, Williams, NA, Birchall, MA & Heyderman, RS 2008, 'Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults', Laryngoscope, vol. 118, no. 3, pp. 459-463. https://doi.org/10.1097/MLG.0b013e31815aedb3
Hobbs CGL, Groves E, Davenport V, Bailey M, Williams NA, Birchall MA et al. Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults. Laryngoscope. 2008 Mar;118(3):459-463. https://doi.org/10.1097/MLG.0b013e31815aedb3
Hobbs, Christopher G L ; Groves, Eleanor ; Davenport, Victoria ; Bailey, Michael ; Williams, Neil A. ; Birchall, Martin A. ; Heyderman, Robert S. / Tonsil T cell immunity to human papillomavirus in the absence of detectable virus in healthy adults. In: Laryngoscope. 2008 ; Vol. 118, No. 3. pp. 459-463.
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