Toll-like receptors 1 and 2 cooperatively mediate immune responses to curli, a common amyloid from enterobacterial biofilms

Çagla Tükel, Jessalyn H. Nishimori, R. Paul Wilson, Maria G. Winter, A. Marijke Keestra, Jos P M Van Putten, Andreas J Baumler

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Responses to host amyloids and curli amyloid fibrils of Escherichia coli and Salmonella enterica serotype Typhimurium are mediated through Toll-like receptor (TLR) 2. Here we show that TLR2 alone was not sufficient for mediating responses to curli. Instead, transfection experiments with human cervical cancer (HeLa) cells and antibody-mediated inhibition of TLR signalling in human macrophage-like (THP-1) cells suggested that TLR2 interacts with TLR1 to recognize curli amyloid fibrils. TLR1/TLR2 also serves as a receptor for tri-acylated lipoproteins, which are produced by E. coli and other Gram-negative bacteria. Despite the presence of multiple TLR1/TLR2 ligands on intact bacterial cells, an inability to produce curli amyloid fibrils markedly reduced the ability of E. coli to induce TLR2-dependent responses in vitro and in vivo. Collectively, our data suggest that curli amyloid fibrils from enterobacterial biofilms significantly contribute to TLR1/TLR2-mediated host responses against intact bacterial cells.

Original languageEnglish (US)
Pages (from-to)1495-1505
Number of pages11
JournalCellular Microbiology
Volume12
Issue number10
DOIs
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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