Abstract
Four sheep-goat chimeras with a goat or sheep sibling having an identical genotype to one of the two component species of cells were tested for tolerance through mixed lymphocyte response (MLR) and skin grafts. None of the four chimeras showed a response to its sib in MLR and three of the four accepted sib skin grafts. This demonstrates that the chimerism exhibited by these animals was sufficient to render the chimera tolerant to antigens expressed by the sib. Two of the four sibs showed positive responses to their chimeric sibs in MLR and two did not; one negative response was expected because the chimera's lymphocytes were essentially all the same species as the sib's. Chimeric skin grafts were partially accepted by two of the four sibs, suggesting the presence of both sheep and goat cells in the skin grafts derived from the chimeras. Two of the four sibs did not accept chimeric skin grafts, possibly due to lack of compatible cells in the graft. Neither differences between an allo- and xenoresponse in MLR nor time differences in the rejection of allo- and xenografts were observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-168 |
| Number of pages | 14 |
| Journal | Journal of Reproductive Immunology |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1993 |
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Keywords
- mixed lymphocyte response (MLR)
- sheep-goat chimeras
- skin grafts
- tolerance
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Reproductive Medicine
- Obstetrics and Gynecology
Cite this
Tolerance of sheep-goat chimeras to their component cells. / Gustafson, Rani A.; Anderson, Gary B.; Bondurant, Robert; Mahi-Brown, Cherrie.
In: Journal of Reproductive Immunology, Vol. 23, No. 2, 01.01.1993, p. 155-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tolerance of sheep-goat chimeras to their component cells
AU - Gustafson, Rani A.
AU - Anderson, Gary B.
AU - Bondurant, Robert
AU - Mahi-Brown, Cherrie
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Four sheep-goat chimeras with a goat or sheep sibling having an identical genotype to one of the two component species of cells were tested for tolerance through mixed lymphocyte response (MLR) and skin grafts. None of the four chimeras showed a response to its sib in MLR and three of the four accepted sib skin grafts. This demonstrates that the chimerism exhibited by these animals was sufficient to render the chimera tolerant to antigens expressed by the sib. Two of the four sibs showed positive responses to their chimeric sibs in MLR and two did not; one negative response was expected because the chimera's lymphocytes were essentially all the same species as the sib's. Chimeric skin grafts were partially accepted by two of the four sibs, suggesting the presence of both sheep and goat cells in the skin grafts derived from the chimeras. Two of the four sibs did not accept chimeric skin grafts, possibly due to lack of compatible cells in the graft. Neither differences between an allo- and xenoresponse in MLR nor time differences in the rejection of allo- and xenografts were observed.
AB - Four sheep-goat chimeras with a goat or sheep sibling having an identical genotype to one of the two component species of cells were tested for tolerance through mixed lymphocyte response (MLR) and skin grafts. None of the four chimeras showed a response to its sib in MLR and three of the four accepted sib skin grafts. This demonstrates that the chimerism exhibited by these animals was sufficient to render the chimera tolerant to antigens expressed by the sib. Two of the four sibs showed positive responses to their chimeric sibs in MLR and two did not; one negative response was expected because the chimera's lymphocytes were essentially all the same species as the sib's. Chimeric skin grafts were partially accepted by two of the four sibs, suggesting the presence of both sheep and goat cells in the skin grafts derived from the chimeras. Two of the four sibs did not accept chimeric skin grafts, possibly due to lack of compatible cells in the graft. Neither differences between an allo- and xenoresponse in MLR nor time differences in the rejection of allo- and xenografts were observed.
KW - mixed lymphocyte response (MLR)
KW - sheep-goat chimeras
KW - skin grafts
KW - tolerance
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UR - http://www.scopus.com/inward/citedby.url?scp=0027419451&partnerID=8YFLogxK
U2 - 10.1016/0165-0378(93)90005-3
DO - 10.1016/0165-0378(93)90005-3
M3 - Article
C2 - 8510078
AN - SCOPUS:0027419451
VL - 23
SP - 155
EP - 168
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
SN - 0165-0378
IS - 2
ER -