Tolerance of sheep-goat chimeras to their component cells

Rani A. Gustafson, Gary B. Anderson, Robert Bondurant, Cherrie Mahi-Brown

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Four sheep-goat chimeras with a goat or sheep sibling having an identical genotype to one of the two component species of cells were tested for tolerance through mixed lymphocyte response (MLR) and skin grafts. None of the four chimeras showed a response to its sib in MLR and three of the four accepted sib skin grafts. This demonstrates that the chimerism exhibited by these animals was sufficient to render the chimera tolerant to antigens expressed by the sib. Two of the four sibs showed positive responses to their chimeric sibs in MLR and two did not; one negative response was expected because the chimera's lymphocytes were essentially all the same species as the sib's. Chimeric skin grafts were partially accepted by two of the four sibs, suggesting the presence of both sheep and goat cells in the skin grafts derived from the chimeras. Two of the four sibs did not accept chimeric skin grafts, possibly due to lack of compatible cells in the graft. Neither differences between an allo- and xenoresponse in MLR nor time differences in the rejection of allo- and xenografts were observed.

Original languageEnglish (US)
Pages (from-to)155-168
Number of pages14
JournalJournal of Reproductive Immunology
Volume23
Issue number2
DOIs
StatePublished - Jan 1 1993

    Fingerprint

Keywords

  • mixed lymphocyte response (MLR)
  • sheep-goat chimeras
  • skin grafts
  • tolerance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this