Tn903 induces inverted duplications in the chromosome of bacteriophage lambda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Specialized transducing strains of bacteriophage lambda have been isolated that carry the transposable kanamycin resistance element, Tn903. Tn903 carries an inverted duplication of 1130 base-pairs flanking the kanamycin resistance gene. Often, when λ::Tn903 particles are infected into bacterial cells, the lambda chromosome is rearranged into a defective lambda plasmid which replicates with the bacterial cell. The formation of the defective plasmids (called Tn903λdv) is most likely induced by the Tn903 insertion itself. This follows from the fact that the novel DNA sequence found in these plasmids, with respect to the ancestral λTn903 chromosome, is always adjacent to the Tn903 element. Physical chromosomal mapping of these plasmids shows that they contain large inverted duplications of lambda sequences situated about the Tn903 insertion. The formation of the Tn903λdv plasmids from the ancestral λTn903 is not dependent on the recombination functions provided through the phage red gene or the host recA gene.

Original languageEnglish (US)
Pages (from-to)1-17
Number of pages17
JournalJournal of Molecular Biology
Volume139
Issue number1
DOIs
StatePublished - May 5 1980
Externally publishedYes

Fingerprint

Chromosome Duplication
Bacteriophage lambda
Plasmids
Kanamycin Resistance
Chromosomes
Genes
Base Pairing
Bacteriophages
Genetic Recombination

ASJC Scopus subject areas

  • Virology

Cite this

Tn903 induces inverted duplications in the chromosome of bacteriophage lambda. / Syvanen, Michael.

In: Journal of Molecular Biology, Vol. 139, No. 1, 05.05.1980, p. 1-17.

Research output: Contribution to journalArticle

@article{c45c3bcfac65422d9194989b7df72b41,
title = "Tn903 induces inverted duplications in the chromosome of bacteriophage lambda",
abstract = "Specialized transducing strains of bacteriophage lambda have been isolated that carry the transposable kanamycin resistance element, Tn903. Tn903 carries an inverted duplication of 1130 base-pairs flanking the kanamycin resistance gene. Often, when λ::Tn903 particles are infected into bacterial cells, the lambda chromosome is rearranged into a defective lambda plasmid which replicates with the bacterial cell. The formation of the defective plasmids (called Tn903λdv) is most likely induced by the Tn903 insertion itself. This follows from the fact that the novel DNA sequence found in these plasmids, with respect to the ancestral λTn903 chromosome, is always adjacent to the Tn903 element. Physical chromosomal mapping of these plasmids shows that they contain large inverted duplications of lambda sequences situated about the Tn903 insertion. The formation of the Tn903λdv plasmids from the ancestral λTn903 is not dependent on the recombination functions provided through the phage red gene or the host recA gene.",
author = "Michael Syvanen",
year = "1980",
month = "5",
day = "5",
doi = "10.1016/0022-2836(80)90112-6",
language = "English (US)",
volume = "139",
pages = "1--17",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Tn903 induces inverted duplications in the chromosome of bacteriophage lambda

AU - Syvanen, Michael

PY - 1980/5/5

Y1 - 1980/5/5

N2 - Specialized transducing strains of bacteriophage lambda have been isolated that carry the transposable kanamycin resistance element, Tn903. Tn903 carries an inverted duplication of 1130 base-pairs flanking the kanamycin resistance gene. Often, when λ::Tn903 particles are infected into bacterial cells, the lambda chromosome is rearranged into a defective lambda plasmid which replicates with the bacterial cell. The formation of the defective plasmids (called Tn903λdv) is most likely induced by the Tn903 insertion itself. This follows from the fact that the novel DNA sequence found in these plasmids, with respect to the ancestral λTn903 chromosome, is always adjacent to the Tn903 element. Physical chromosomal mapping of these plasmids shows that they contain large inverted duplications of lambda sequences situated about the Tn903 insertion. The formation of the Tn903λdv plasmids from the ancestral λTn903 is not dependent on the recombination functions provided through the phage red gene or the host recA gene.

AB - Specialized transducing strains of bacteriophage lambda have been isolated that carry the transposable kanamycin resistance element, Tn903. Tn903 carries an inverted duplication of 1130 base-pairs flanking the kanamycin resistance gene. Often, when λ::Tn903 particles are infected into bacterial cells, the lambda chromosome is rearranged into a defective lambda plasmid which replicates with the bacterial cell. The formation of the defective plasmids (called Tn903λdv) is most likely induced by the Tn903 insertion itself. This follows from the fact that the novel DNA sequence found in these plasmids, with respect to the ancestral λTn903 chromosome, is always adjacent to the Tn903 element. Physical chromosomal mapping of these plasmids shows that they contain large inverted duplications of lambda sequences situated about the Tn903 insertion. The formation of the Tn903λdv plasmids from the ancestral λTn903 is not dependent on the recombination functions provided through the phage red gene or the host recA gene.

UR - http://www.scopus.com/inward/record.url?scp=0019317102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019317102&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(80)90112-6

DO - 10.1016/0022-2836(80)90112-6

M3 - Article

C2 - 6267289

AN - SCOPUS:0019317102

VL - 139

SP - 1

EP - 17

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -