Titration of HPV-11 infectivity and antibody neutralization can be measured in vitro

Lloyd H Smith, C. Foster, M. E. Hitchcock, Gary S Leiserowitz, K. Hall, Roslyn Rivkah Isseroff, N. D. Christensen, J. W. Kreider

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Human papillomavirus type 11 (HPV-11), produced from the athymic mouse xenograft system, was shown to infect cultured neonatal human foreskin keratinocytes and the HaCaT keratinocyte cell line in vitro. Infection was documented by the appearance of HPV-11-specific spliced mRNA, detected by reverse transcriptase-polymerase chain reaction. Purified HPV-11 virions at concentrations of approximately 107 particles/ml could successfully evoke infection in this system. Infection was completely abrogated by preincubation of the HPV-11 inoculum with mouse anti-HPV-11 monoclonal antibodies, experimentally immunized animal sera, or sera of human patients with HPV infection. Concurrent detection of cellular mRNA for the β-actin gene, also by reverse transcriptase-polymerase chain reaction, provided an internal control confirming RNA recovery and successful reverse transcriptase-polymerase chain reaction. Using this approach, it was possible to determine semiquantitative titers for test solutions of HPV-11-neutralizing antibodies. The in vitro system for HPV-11 infectivity and neutralization may be useful in the study of the immune response to HPV-11 infection or immunization in patients.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalJournal of Investigative Dermatology
Volume105
Issue number3
StatePublished - 1995

Keywords

  • reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Dermatology

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