Tissue vitamin B-12 assay by a radioisotope dilution technique

Susan V. Van Tonder, Jack Metz, Ralph Green

Research output: Contribution to journalArticle

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Abstract

A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modification using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co]B-12 administered to bats. Results using the RID assay in 16 human and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results. Lower results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues.

Original languageEnglish (US)
Pages (from-to)285-296
Number of pages12
JournalClinica Chimica Acta
Volume63
Issue number3
DOIs
StatePublished - Sep 16 1975
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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