TY - JOUR
T1 - Tissue vitamin B-12 assay by a radioisotope dilution technique
AU - Van Tonder, Susan V.
AU - Metz, Jack
AU - Green, Ralph
PY - 1975/9/16
Y1 - 1975/9/16
N2 - A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modification using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co]B-12 administered to bats. Results using the RID assay in 16 human and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results. Lower results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues.
AB - A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modification using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co]B-12 administered to bats. Results using the RID assay in 16 human and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results. Lower results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues.
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U2 - 10.1016/0009-8981(75)90049-2
DO - 10.1016/0009-8981(75)90049-2
M3 - Article
C2 - 1100288
AN - SCOPUS:0016593734
VL - 63
SP - 285
EP - 296
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 3
ER -