Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array

Doris Lam, Heather A. Enright, Jose Cadena, Sandra K.G. Peters, Ana Paula Sales, Joanne J. Osburn, David A. Soscia, Kristen S. Kulp, Elizabeth K. Wheeler, Nicholas O Fischer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The brain’s extracellular matrix (ECM) is a macromolecular network composed of glycosaminoglycans, proteoglycans, glycoproteins, and fibrous proteins. In vitro studies often use purified ECM proteins for cell culture coatings, however these may not represent the molecular complexity and heterogeneity of the brain’s ECM. To address this, we compared neural network activity (over 30 days in vitro) from primary neurons co-cultured with glia grown on ECM coatings from decellularized brain tissue (bECM) or MaxGel, a non-tissue-specific ECM. Cells were grown on a multi-electrode array (MEA) to enable noninvasive long-term interrogation of neuronal networks. In general, the presence of ECM accelerated the formation of networks without affecting the inherent network properties. However, specific features of network activity were dependent on the type of ECM: bECM enhanced network activity over a greater region of the MEA whereas MaxGel increased network burst rate associated with robust synaptophysin expression. These differences in network activity were not attributable to cellular composition, glial proliferation, or astrocyte phenotypes, which remained constant across experimental conditions. Collectively, the addition of ECM to neuronal cultures represents a reliable method to accelerate the development of mature neuronal networks, providing a means to enhance throughput for routine evaluation of neurotoxins and novel therapeutics.

Original languageEnglish (US)
Article number4159
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

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Coculture Techniques
Neuroglia
Extracellular Matrix
Electrodes
Neurons
Brain
Scleroproteins
Synaptophysin
Extracellular Matrix Proteins
Neurotoxins
Proteoglycans
Glycosaminoglycans
Astrocytes
Glycoproteins
Cell Culture Techniques
Phenotype

ASJC Scopus subject areas

  • General

Cite this

Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array. / Lam, Doris; Enright, Heather A.; Cadena, Jose; Peters, Sandra K.G.; Sales, Ana Paula; Osburn, Joanne J.; Soscia, David A.; Kulp, Kristen S.; Wheeler, Elizabeth K.; Fischer, Nicholas O.

In: Scientific Reports, Vol. 9, No. 1, 4159, 01.12.2019.

Research output: Contribution to journalArticle

Lam, Doris ; Enright, Heather A. ; Cadena, Jose ; Peters, Sandra K.G. ; Sales, Ana Paula ; Osburn, Joanne J. ; Soscia, David A. ; Kulp, Kristen S. ; Wheeler, Elizabeth K. ; Fischer, Nicholas O. / Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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