Time-resolved SERS for characterizing extracellular vesicles

Tatu Rojalin; Heikki Saari; Petter Somersalo; Saara Laitinen; Mikko Turunen; Tapani Viitala; Sebastian Wachsmann-Hogiu; Zachary J. Smith; Marjo Yliperttula

doi:10.1117/12.2271865

Time-resolved SERS for characterizing extracellular vesicles

Tatu Rojalin, Heikki Saari, Petter Somersalo, Saara Laitinen, Mikko Turunen, Tapani Viitala, Sebastian Wachsmann-Hogiu, Zachary J. Smith, Marjo Yliperttula

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The aim of this work is to develop a platform for characterizing extracellular vesicles (EV) by using gold-polymer nanopillar SERS arrays simultaneously circumventing the photoluminescence-related disadvantages of Raman with a time-resolved approach. EVs are rich of biochemical information reporting of, for example, diseased state of the biological system. Currently, straightforward, label-free and fast EV characterization methods with low sample consumption are warranted. In this study, SERS spectra of red blood cell and platelet derived EVs were successfully measured and their biochemical contents analyzed using multivariate data analysis techniques. The developed platform could be conveniently used for EV analytics in general.

Original language	English (US)
Title of host publication	Plasmonics in Biology and Medicine XIV
Publisher	SPIE
Volume	10080
ISBN (Electronic)	9781510606012
DOIs	https://doi.org/10.1117/12.2271865
State	Published - 2017
Externally published	Yes
Event	Plasmonics in Biology and Medicine XIV - San Francisco, United States Duration: Jan 30 2017 → …

Other

Other	Plasmonics in Biology and Medicine XIV
Country	United States
City	San Francisco
Period	1/30/17 → …

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2271865

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Time-resolved SERS for characterizing extracellular vesicles. / Rojalin, Tatu; Saari, Heikki; Somersalo, Petter; Laitinen, Saara; Turunen, Mikko; Viitala, Tapani; Wachsmann-Hogiu, Sebastian; Smith, Zachary J.; Yliperttula, Marjo.

Plasmonics in Biology and Medicine XIV. Vol. 10080 SPIE, 2017. 100800U.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Rojalin, T, Saari, H, Somersalo, P, Laitinen, S, Turunen, M, Viitala, T, Wachsmann-Hogiu, S, Smith, ZJ & Yliperttula, M 2017, Time-resolved SERS for characterizing extracellular vesicles. in Plasmonics in Biology and Medicine XIV. vol. 10080, 100800U, SPIE, Plasmonics in Biology and Medicine XIV, San Francisco, United States, 1/30/17. https://doi.org/10.1117/12.2271865

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