Abstract
Objective: The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC). Study Design: Cross-sectional study. Setting: University-based medical center. Subjects and Methods: Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337 nm, 700-picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time-domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined. Results: Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660 nm. Multiple time-resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390 nm: 1.7 ± 0.06 ns [not significant] for normal and 1.3 ± 0.06 ns for tumor, P = 0.0025) and the second-order Laguerre expansion coefficient (LEC-2) (i.e., at 460 nm: 0.135 ± 0.001 for normal and 0.155 ± 0.007 for tumor, P < 0.05). Conclusion: These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR-LIFS has potential as a noninvasive diagnostic technique for HNSCC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 838-844 |
| Number of pages | 7 |
| Journal | Otolaryngology - Head and Neck Surgery |
| Volume | 142 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2010 |
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ASJC Scopus subject areas
- Otorhinolaryngology
- Surgery
Cite this
Time-resolved laser-induced fluorescence spectroscopy as a diagnostic instrument in head and neck carcinoma. / Meier, Jeremy D.; Xie, Hongtao; Sun, Yang; Sun, Yinghua; Hatami, Nisa; Poirier, Brian; Marcu, Laura; Farwell, D Gregory.
In: Otolaryngology - Head and Neck Surgery, Vol. 142, No. 6, 06.2010, p. 838-844.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-resolved laser-induced fluorescence spectroscopy as a diagnostic instrument in head and neck carcinoma
AU - Meier, Jeremy D.
AU - Xie, Hongtao
AU - Sun, Yang
AU - Sun, Yinghua
AU - Hatami, Nisa
AU - Poirier, Brian
AU - Marcu, Laura
AU - Farwell, D Gregory
PY - 2010/6
Y1 - 2010/6
N2 - Objective: The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC). Study Design: Cross-sectional study. Setting: University-based medical center. Subjects and Methods: Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337 nm, 700-picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time-domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined. Results: Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660 nm. Multiple time-resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390 nm: 1.7 ± 0.06 ns [not significant] for normal and 1.3 ± 0.06 ns for tumor, P = 0.0025) and the second-order Laguerre expansion coefficient (LEC-2) (i.e., at 460 nm: 0.135 ± 0.001 for normal and 0.155 ± 0.007 for tumor, P < 0.05). Conclusion: These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR-LIFS has potential as a noninvasive diagnostic technique for HNSCC.
AB - Objective: The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC). Study Design: Cross-sectional study. Setting: University-based medical center. Subjects and Methods: Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337 nm, 700-picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time-domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined. Results: Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660 nm. Multiple time-resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390 nm: 1.7 ± 0.06 ns [not significant] for normal and 1.3 ± 0.06 ns for tumor, P = 0.0025) and the second-order Laguerre expansion coefficient (LEC-2) (i.e., at 460 nm: 0.135 ± 0.001 for normal and 0.155 ± 0.007 for tumor, P < 0.05). Conclusion: These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR-LIFS has potential as a noninvasive diagnostic technique for HNSCC.
UR - http://www.scopus.com/inward/record.url?scp=77952252717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952252717&partnerID=8YFLogxK
U2 - 10.1016/j.otohns.2010.02.005
DO - 10.1016/j.otohns.2010.02.005
M3 - Article
C2 - 20493355
AN - SCOPUS:77952252717
VL - 142
SP - 838
EP - 844
JO - Otolaryngology - Head and Neck Surgery (United States)
JF - Otolaryngology - Head and Neck Surgery (United States)
SN - 0194-5998
IS - 6
ER -