Time-resolved fluorescence spectroscopy for the diagnosis of oral lichen planus

D. Gorpas, P. Davari, J. Bec, Maxwell A Fung, Laura Marcu, D Gregory Farwell, Nasim Fazel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Lichen planus (LP) is a T-cell mediated autoimmune disorder of unknown aetiology that affects the skin, nails, oral and genital mucous membranes. Conventionally, oral LP (OLP) is diagnosed through clinical assessment and histopathological confirmation by oral biopsy. Aim: To explore the use of time-resolved fluorescence spectroscopy (TRFS) to detect fluorescence lifetime changes between lesional OLP and perilesional normal mucosa. Methods: In this pilot study, measurements of lesional and perilesional buccal and mouth floor mucosa were conducted in vivo with a TRFS system. Histopathological findings were consistent with OLP in 8 out of 10 patients biopsied. Two patients with histopathological diagnoses of frictional hyperkeratosis and oral candidiasis, respectively, were excluded from the study. Results: Our preliminary data show that lifetime values in the 360-560 nm spectral range indicate a significant differentiation between normal and diseased tissue. In contrast to the standard oral biopsy procedure, this technique is noninvasive, painless, time-efficient and safe. Conclusions: Future studies are needed to better elucidate the diagnostic capability of TRFS and to further explore the sources of fluorescence contrast. This pilot study suggests that, based on fluorescence lifetime parameters, TRFS is a very promising technology for the development of a novel OLP diagnostic technique.

Original languageEnglish (US)
JournalClinical and Experimental Dermatology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Oral Lichen Planus
Fluorescence Spectrometry
Fluorescence
Mucous Membrane
Oral Candidiasis
Biopsy
Mouth Floor
Lichen Planus
Cheek
Mouth Mucosa
Nails
Technology
T-Lymphocytes
Skin

ASJC Scopus subject areas

  • Dermatology

Cite this

Time-resolved fluorescence spectroscopy for the diagnosis of oral lichen planus. / Gorpas, D.; Davari, P.; Bec, J.; Fung, Maxwell A; Marcu, Laura; Farwell, D Gregory; Fazel, Nasim.

In: Clinical and Experimental Dermatology, 01.01.2018.

Research output: Contribution to journalArticle

@article{b1bd9a38e16746a4bd21036c53ca3680,
title = "Time-resolved fluorescence spectroscopy for the diagnosis of oral lichen planus",
abstract = "Background: Lichen planus (LP) is a T-cell mediated autoimmune disorder of unknown aetiology that affects the skin, nails, oral and genital mucous membranes. Conventionally, oral LP (OLP) is diagnosed through clinical assessment and histopathological confirmation by oral biopsy. Aim: To explore the use of time-resolved fluorescence spectroscopy (TRFS) to detect fluorescence lifetime changes between lesional OLP and perilesional normal mucosa. Methods: In this pilot study, measurements of lesional and perilesional buccal and mouth floor mucosa were conducted in vivo with a TRFS system. Histopathological findings were consistent with OLP in 8 out of 10 patients biopsied. Two patients with histopathological diagnoses of frictional hyperkeratosis and oral candidiasis, respectively, were excluded from the study. Results: Our preliminary data show that lifetime values in the 360-560 nm spectral range indicate a significant differentiation between normal and diseased tissue. In contrast to the standard oral biopsy procedure, this technique is noninvasive, painless, time-efficient and safe. Conclusions: Future studies are needed to better elucidate the diagnostic capability of TRFS and to further explore the sources of fluorescence contrast. This pilot study suggests that, based on fluorescence lifetime parameters, TRFS is a very promising technology for the development of a novel OLP diagnostic technique.",
author = "D. Gorpas and P. Davari and J. Bec and Fung, {Maxwell A} and Laura Marcu and Farwell, {D Gregory} and Nasim Fazel",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/ced.13404",
language = "English (US)",
journal = "Clinical and Experimental Dermatology",
issn = "0307-6938",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Time-resolved fluorescence spectroscopy for the diagnosis of oral lichen planus

AU - Gorpas, D.

AU - Davari, P.

AU - Bec, J.

AU - Fung, Maxwell A

AU - Marcu, Laura

AU - Farwell, D Gregory

AU - Fazel, Nasim

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Lichen planus (LP) is a T-cell mediated autoimmune disorder of unknown aetiology that affects the skin, nails, oral and genital mucous membranes. Conventionally, oral LP (OLP) is diagnosed through clinical assessment and histopathological confirmation by oral biopsy. Aim: To explore the use of time-resolved fluorescence spectroscopy (TRFS) to detect fluorescence lifetime changes between lesional OLP and perilesional normal mucosa. Methods: In this pilot study, measurements of lesional and perilesional buccal and mouth floor mucosa were conducted in vivo with a TRFS system. Histopathological findings were consistent with OLP in 8 out of 10 patients biopsied. Two patients with histopathological diagnoses of frictional hyperkeratosis and oral candidiasis, respectively, were excluded from the study. Results: Our preliminary data show that lifetime values in the 360-560 nm spectral range indicate a significant differentiation between normal and diseased tissue. In contrast to the standard oral biopsy procedure, this technique is noninvasive, painless, time-efficient and safe. Conclusions: Future studies are needed to better elucidate the diagnostic capability of TRFS and to further explore the sources of fluorescence contrast. This pilot study suggests that, based on fluorescence lifetime parameters, TRFS is a very promising technology for the development of a novel OLP diagnostic technique.

AB - Background: Lichen planus (LP) is a T-cell mediated autoimmune disorder of unknown aetiology that affects the skin, nails, oral and genital mucous membranes. Conventionally, oral LP (OLP) is diagnosed through clinical assessment and histopathological confirmation by oral biopsy. Aim: To explore the use of time-resolved fluorescence spectroscopy (TRFS) to detect fluorescence lifetime changes between lesional OLP and perilesional normal mucosa. Methods: In this pilot study, measurements of lesional and perilesional buccal and mouth floor mucosa were conducted in vivo with a TRFS system. Histopathological findings were consistent with OLP in 8 out of 10 patients biopsied. Two patients with histopathological diagnoses of frictional hyperkeratosis and oral candidiasis, respectively, were excluded from the study. Results: Our preliminary data show that lifetime values in the 360-560 nm spectral range indicate a significant differentiation between normal and diseased tissue. In contrast to the standard oral biopsy procedure, this technique is noninvasive, painless, time-efficient and safe. Conclusions: Future studies are needed to better elucidate the diagnostic capability of TRFS and to further explore the sources of fluorescence contrast. This pilot study suggests that, based on fluorescence lifetime parameters, TRFS is a very promising technology for the development of a novel OLP diagnostic technique.

UR - http://www.scopus.com/inward/record.url?scp=85041910852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041910852&partnerID=8YFLogxK

U2 - 10.1111/ced.13404

DO - 10.1111/ced.13404

M3 - Article

C2 - 29436013

AN - SCOPUS:85041910852

JO - Clinical and Experimental Dermatology

JF - Clinical and Experimental Dermatology

SN - 0307-6938

ER -