TY - JOUR
T1 - Time-resolved fluorescence of human aortic wall
T2 - Use for improved identification of atherosclerotic lesions
AU - Maarek, Jean Michel I
AU - Marcu, Laura
AU - Fishbein, Michael C.
AU - Grundfest, Warren S.
PY - 2000
Y1 - 2000
N2 - Background and Objective: This study characterized aortic time-resolved fluorescence spectra for stratified levels of atherosclerosis and proposed interpretation of spectrotemporal variations in terms of histologic changes. Study Design/Materials and Methods: Fluorescence emission transients were measured at 370-510 nm (337 nm excitation) on 94 excised human aortic samples, ranging from normal to advanced fibrous atherosclerotic lesion. Global analysis yielded a three-exponential approximation of the time-resolved spectra from which average lifetime and decay-associated spectra were derived. Results: Average lifetime at 390 nm gradually increased from 2.4 ± 0.1 nsec (normal aorta) to 3.9 ± 0.1 nsec (advanced lesion). Fluorescence intensity was markedly decreased above 430 nm in intermediate and advanced lesions. Spectral intensity associated with the intermediate decay increased at 470-490 nm for early and intermediate lipid-rich lesions. Conclusion: Time-resolved fluorescence spectra of aortic samples presented distinctive features for each atherosclerotic lesion type, which could serve as characteristic markers for optical analysis of the aortic wall. (C) 2000 Wiley-Liss, Inc.
AB - Background and Objective: This study characterized aortic time-resolved fluorescence spectra for stratified levels of atherosclerosis and proposed interpretation of spectrotemporal variations in terms of histologic changes. Study Design/Materials and Methods: Fluorescence emission transients were measured at 370-510 nm (337 nm excitation) on 94 excised human aortic samples, ranging from normal to advanced fibrous atherosclerotic lesion. Global analysis yielded a three-exponential approximation of the time-resolved spectra from which average lifetime and decay-associated spectra were derived. Results: Average lifetime at 390 nm gradually increased from 2.4 ± 0.1 nsec (normal aorta) to 3.9 ± 0.1 nsec (advanced lesion). Fluorescence intensity was markedly decreased above 430 nm in intermediate and advanced lesions. Spectral intensity associated with the intermediate decay increased at 470-490 nm for early and intermediate lipid-rich lesions. Conclusion: Time-resolved fluorescence spectra of aortic samples presented distinctive features for each atherosclerotic lesion type, which could serve as characteristic markers for optical analysis of the aortic wall. (C) 2000 Wiley-Liss, Inc.
KW - Atherosclerosis
KW - Decay-associated spectra
KW - Fluorescence spectroscopy
KW - Global analysis
KW - Lifetime
KW - Nitrogen laser
KW - Optical biopsy
KW - Time-dependent fluorescence decay
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U2 - 10.1002/1096-9101(2000)27:3<241::AID-LSM6>3.0.CO;2-0
DO - 10.1002/1096-9101(2000)27:3<241::AID-LSM6>3.0.CO;2-0
M3 - Article
C2 - 11013386
AN - SCOPUS:0033795565
VL - 27
SP - 241
EP - 254
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
SN - 0196-8092
IS - 3
ER -