Time-of-flight resolved light field fluctuations reveal deep human tissue physiology

Oybek Kholiqov; Wenjun Zhou; Tingwei Zhang; V. N. Du Le; Vivek J. Srinivasan

doi:10.1038/s41467-019-14228-5

Time-of-flight resolved light field fluctuations reveal deep human tissue physiology

Oybek Kholiqov, Wenjun Zhou, Tingwei Zhang, V. N. Du Le, Vivek J. Srinivasan

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Red blood cells (RBCs) transport oxygen to tissues and remove carbon dioxide. Diffuse optical flowmetry (DOF) assesses deep tissue RBC dynamics by measuring coherent fluctuations of multiply scattered near-infrared light intensity. While classical DOF measurements empirically correlate with blood flow, they remain far-removed from light scattering physics and difficult to interpret in layered media. To advance DOF measurements closer to the physics, here we introduce an interferometric technique, surmounting challenges of bulk motion to apply it in awake humans. We reveal two measurement dimensions: optical phase, and time-of-flight (TOF), the latter with 22 picosecond resolution. With this multidimensional data, we directly confirm the unordered, or Brownian, nature of optically probed RBC dynamics typically assumed in classical DOF. We illustrate how incorrect absorption assumptions, anisotropic RBC scattering, and layered tissues may confound classical DOF. By comparison, our direct method enables accurate and comprehensive assessment of blood flow dynamics in humans.

Original language	English (US)
Article number	391
Journal	Nature communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-14228-5
State	Published - Dec 1 2020

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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