Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence

Sonny Ly, Gregory McNerney, Samantha Fore, James W Chan, Thomas R Huser

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We demonstrate time-gated confocal imaging as a means to separate coherent anti-Stokes Raman scattering (CARS) microscopy data from multi-photon excited endogenous fluorescence in tissue. CARS is a quasi-instantaneous process and its signal decay time is only limited by the system's instrument response function (IRF). Signals due to two-photon-excited (TPE) tissue autofluorescence with excited state lifetimes on the nanosecond scale can be identified and separated from the CARS signal by employing time-gating techniques. We demonstrate this improved contrast on the example of CARS microscopy of intact roots of plant seedlings as well as on rat arterial tissue.

Original languageEnglish (US)
Pages (from-to)16839-16851
Number of pages13
JournalOptics Express
Volume15
Issue number25
StatePublished - Dec 12 2007

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counting
Raman spectra
microscopy
photons
rats
life (durability)
fluorescence
decay
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. / Ly, Sonny; McNerney, Gregory; Fore, Samantha; Chan, James W; Huser, Thomas R.

In: Optics Express, Vol. 15, No. 25, 12.12.2007, p. 16839-16851.

Research output: Contribution to journalArticle

Ly, Sonny ; McNerney, Gregory ; Fore, Samantha ; Chan, James W ; Huser, Thomas R. / Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence. In: Optics Express. 2007 ; Vol. 15, No. 25. pp. 16839-16851.
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