Time gated detection of europium nanoparticles in a micro channel based environmental immunoassay

Shi Che Chen, R. Perron, Dosi Dosev, Ian Kennedy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Using MEMs (Micro Electro Mechanical system) fabrication techniques, it is possible make a micro-sized instrument for optical detection of trace amounts of chemical species in aqueous solutions. The redemitting EU 2O 3 nanoparticle is suitable for a biolabel for such species because of its long fluorescence lifetime and narrow emission bandwidth. The europium nanoparticles are excited by a laser pulse. Their long-lived emission allows the detected signal to be separated from the laser pulse both spectrally and temporally. The background signal can also be eliminated in this manner. The instrument we present, is assembled with silicon and glass layers with a 200μm deep channel. A Nd:YAG pumped optical parametric oscillator (OPO) is used as the excitation source. The measurement sensitivities using two detectors, a PMT (Photo Multiplier Tube) and an APD (Avalanche Photodiode), are compared. The underlying fundamental principles and the micro-fabrication steps for the instrument and detection are discussed.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.V. Nicolau, U.R. Muller, J.M. Dell
Pages186-196
Number of pages11
Volume5275
DOIs
StatePublished - 2004
EventBioMEMS and Nanotechnology - Perth, WA, Australia
Duration: Dec 10 2003Dec 12 2003

Other

OtherBioMEMS and Nanotechnology
CountryAustralia
CityPerth, WA
Period12/10/0312/12/03

Keywords

  • Europium
  • Fluorescence
  • Lifetime
  • MEMS
  • Nanoparticles

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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