Time course of gastrointestinal tract permeability to chromium 51- labeled ethylenediaminetetraacetate in healthy dogs

Stanley L Marks, D. A. Williams

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives - To establish values for gastrointestinal tract permeation by chromium 51-labeled ethylenediaminetetraacetate (51Cr-labeled EDTA) in healthy adult dogs, and to evaluate the time course for 51Cr-labeled EDTA absorption over a 24-hour period after its administration, in an effort to define a shorter, more practical collection method. Animals - 6 healthy adult mixed-breed dogs. Procedure - After an 18-hour nonfeeding period, each dog was given a solution containing 50 μCi of 51Cr-labeled EDTA in deionized water (10 ml/kg of body weight) by stomach tube. Complete urine collection was done at 2, 4, 6, and 24 hours after 51Cr-labeled EDTA administration. Five-milliliter samples of urine were counted for 15 minutes in a gamma counter, and radioactivity in urine was expressed as a percentage of the orally administered dose. Results - Median (range) 24-hour urinary recovery of 51Cr-labeled EDTA after 24 hours was 15.1 (12.7 to 20.3)%. Urine collected at 2, 4, and 6 hours contained 1.0 (0.2 to 3.5)%, 6.5 (2.2. to 8.7)%, and 10.0 (8.1 to 11.7)% of the administered 51Cr-labeled EDTA, respectively. Urine passed during the first 6 hours contained, on average, 67 (54 to 77)% of the total 24-hour urine recovery. Conclusions - 6-hour urinary recovery of 51Cr-EDTA provides a potential alternative to 24-hour recovery. This shorter collection period may more specifically reflect small intestinal permeability.

Original languageEnglish (US)
Pages (from-to)1113-1115
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume59
Issue number9
StatePublished - 1998

Fingerprint

Chromium
chromium
Edetic Acid
gastrointestinal system
Gastrointestinal Tract
Permeability
permeability
urine
Dogs
dogs
Urine
administered dose
Urine Specimen Collection
stomach
Radioactivity
Stomach
Body Weight
body weight
Water
animals

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{1b9dfdaa105b40d5ab95d3a739f686f2,
title = "Time course of gastrointestinal tract permeability to chromium 51- labeled ethylenediaminetetraacetate in healthy dogs",
abstract = "Objectives - To establish values for gastrointestinal tract permeation by chromium 51-labeled ethylenediaminetetraacetate (51Cr-labeled EDTA) in healthy adult dogs, and to evaluate the time course for 51Cr-labeled EDTA absorption over a 24-hour period after its administration, in an effort to define a shorter, more practical collection method. Animals - 6 healthy adult mixed-breed dogs. Procedure - After an 18-hour nonfeeding period, each dog was given a solution containing 50 μCi of 51Cr-labeled EDTA in deionized water (10 ml/kg of body weight) by stomach tube. Complete urine collection was done at 2, 4, 6, and 24 hours after 51Cr-labeled EDTA administration. Five-milliliter samples of urine were counted for 15 minutes in a gamma counter, and radioactivity in urine was expressed as a percentage of the orally administered dose. Results - Median (range) 24-hour urinary recovery of 51Cr-labeled EDTA after 24 hours was 15.1 (12.7 to 20.3){\%}. Urine collected at 2, 4, and 6 hours contained 1.0 (0.2 to 3.5){\%}, 6.5 (2.2. to 8.7){\%}, and 10.0 (8.1 to 11.7){\%} of the administered 51Cr-labeled EDTA, respectively. Urine passed during the first 6 hours contained, on average, 67 (54 to 77){\%} of the total 24-hour urine recovery. Conclusions - 6-hour urinary recovery of 51Cr-EDTA provides a potential alternative to 24-hour recovery. This shorter collection period may more specifically reflect small intestinal permeability.",
author = "Marks, {Stanley L} and Williams, {D. A.}",
year = "1998",
language = "English (US)",
volume = "59",
pages = "1113--1115",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "9",

}

TY - JOUR

T1 - Time course of gastrointestinal tract permeability to chromium 51- labeled ethylenediaminetetraacetate in healthy dogs

AU - Marks, Stanley L

AU - Williams, D. A.

PY - 1998

Y1 - 1998

N2 - Objectives - To establish values for gastrointestinal tract permeation by chromium 51-labeled ethylenediaminetetraacetate (51Cr-labeled EDTA) in healthy adult dogs, and to evaluate the time course for 51Cr-labeled EDTA absorption over a 24-hour period after its administration, in an effort to define a shorter, more practical collection method. Animals - 6 healthy adult mixed-breed dogs. Procedure - After an 18-hour nonfeeding period, each dog was given a solution containing 50 μCi of 51Cr-labeled EDTA in deionized water (10 ml/kg of body weight) by stomach tube. Complete urine collection was done at 2, 4, 6, and 24 hours after 51Cr-labeled EDTA administration. Five-milliliter samples of urine were counted for 15 minutes in a gamma counter, and radioactivity in urine was expressed as a percentage of the orally administered dose. Results - Median (range) 24-hour urinary recovery of 51Cr-labeled EDTA after 24 hours was 15.1 (12.7 to 20.3)%. Urine collected at 2, 4, and 6 hours contained 1.0 (0.2 to 3.5)%, 6.5 (2.2. to 8.7)%, and 10.0 (8.1 to 11.7)% of the administered 51Cr-labeled EDTA, respectively. Urine passed during the first 6 hours contained, on average, 67 (54 to 77)% of the total 24-hour urine recovery. Conclusions - 6-hour urinary recovery of 51Cr-EDTA provides a potential alternative to 24-hour recovery. This shorter collection period may more specifically reflect small intestinal permeability.

AB - Objectives - To establish values for gastrointestinal tract permeation by chromium 51-labeled ethylenediaminetetraacetate (51Cr-labeled EDTA) in healthy adult dogs, and to evaluate the time course for 51Cr-labeled EDTA absorption over a 24-hour period after its administration, in an effort to define a shorter, more practical collection method. Animals - 6 healthy adult mixed-breed dogs. Procedure - After an 18-hour nonfeeding period, each dog was given a solution containing 50 μCi of 51Cr-labeled EDTA in deionized water (10 ml/kg of body weight) by stomach tube. Complete urine collection was done at 2, 4, 6, and 24 hours after 51Cr-labeled EDTA administration. Five-milliliter samples of urine were counted for 15 minutes in a gamma counter, and radioactivity in urine was expressed as a percentage of the orally administered dose. Results - Median (range) 24-hour urinary recovery of 51Cr-labeled EDTA after 24 hours was 15.1 (12.7 to 20.3)%. Urine collected at 2, 4, and 6 hours contained 1.0 (0.2 to 3.5)%, 6.5 (2.2. to 8.7)%, and 10.0 (8.1 to 11.7)% of the administered 51Cr-labeled EDTA, respectively. Urine passed during the first 6 hours contained, on average, 67 (54 to 77)% of the total 24-hour urine recovery. Conclusions - 6-hour urinary recovery of 51Cr-EDTA provides a potential alternative to 24-hour recovery. This shorter collection period may more specifically reflect small intestinal permeability.

UR - http://www.scopus.com/inward/record.url?scp=0031659921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031659921&partnerID=8YFLogxK

M3 - Article

C2 - 9736386

AN - SCOPUS:0031659921

VL - 59

SP - 1113

EP - 1115

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 9

ER -