Time- and dose-dependent differential upregulation of three genes by 17β-estradiol in endothelial cells

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19 Scopus citations

Abstract

The purpose of this study was to identify genetic targets in the vasculature for estrogen by profiling genes expressed in female human aortic endothelial cells exposed to various doses of 17β-estradiol at differing concentrations and for differing periods of time. Our approach employed a RT-PCR-based cloning strategy of DNA differential display analysis, with differential expression verified by semiquantitative PCR performed with gene-specific primers. A significant increase in mRNA expression in response to 17β-estradiol was observed for the following three genes: aldose reductase (3.4-fold), caspase homologue-α protein (4.2-fold), and plasminogen activator inhibitor-1 intron e (2.3-fold). For all three upregulated genes, estradiol-induced upregulation occurred with a similar time course and temporally clustered to the first 24 h after hormone treatment. In addition, the effect of estradiol dose on gene expression was consistent and occurred at physiological concentrations. Our results describe previously uncharacterized estradiol-sensitive time- and dose-dependent regulation of genes with potential importance to vascular function in human endothelial cells.

Original languageEnglish (US)
Pages (from-to)1064-1073
Number of pages10
JournalJournal of Applied Physiology
Volume92
Issue number3
StatePublished - 2002

Keywords

  • Aldose reductase
  • Caspase homologue
  • Cell culture
  • Differential display
  • Endothelial
  • Estradiol
  • Estrogen
  • Gene expression
  • MRNA
  • Plasminogen activator inhibitor-1
  • RT-PCR
  • Vascular

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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