tie-1 protein tyrosine kinase

A novel independent prognostic marker for gastric cancer

Wen Chang Lin, Anna F Y Li, Chin Wen Chi, Wen Wei Chung, Chen Lung Huang, Wing Yiu Lui, Hsing-Jien Kung, Chew Wun Wu

Research output: Contribution to journalArticle

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Abstract

Protein tyrosine kinases (PTKs) are a major class of proto-oncogenes that are involved in tumor progression. The purpose of this study was to establish a comprehensive PTK expression profile in gastric cancers, with the objective of identifying possible biomarkers for gastric cancer progression. We have designed degenerate primers according to the consensus catalytic motifs to amplify PTK molecules from gastric cancers by reverse transcriptase-PCR methods. The PTK expression profile was established by sequencing analysis of the cloned PCR products. We have identified 17 PTKs from a gastric adenocarcinoma. Two receptor PTKs, tie-1 and axl, were selected for in situ immunohistochemistry studies because of their higher expression level and their described roles in adhesion, invasion, and angiogenesis. Among the 97 gastric adenocarcinoma tissues examined, we observed positive immunohistochemical staining of tie-1 PTK in 69 and positive staining of axl kinase in 71 tissues. Statistical analysis with clinicopathological features indicates that tie-1 kinase expression is inversely correlated with patients' survival, whereas axl fails to show similar clinical significance. Our results illustrate the utility of tyrosine kinase gene family profiling in human gastric cancers and show that tie-1 tyrosine kinase may serve as a novel independent prognostic marker for gastric adenocarcinoma patients.

Original languageEnglish (US)
Pages (from-to)1745-1751
Number of pages7
JournalClinical Cancer Research
Volume5
Issue number7
StatePublished - Jul 1999
Externally publishedYes

Fingerprint

Protein-Tyrosine Kinases
Stomach Neoplasms
Stomach
Adenocarcinoma
Phosphotransferases
Staining and Labeling
Proto-Oncogenes
Receptor Protein-Tyrosine Kinases
Reverse Transcriptase Polymerase Chain Reaction
Biomarkers
Immunohistochemistry
Polymerase Chain Reaction
Survival
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lin, W. C., Li, A. F. Y., Chi, C. W., Chung, W. W., Huang, C. L., Lui, W. Y., ... Wu, C. W. (1999). tie-1 protein tyrosine kinase: A novel independent prognostic marker for gastric cancer. Clinical Cancer Research, 5(7), 1745-1751.

tie-1 protein tyrosine kinase : A novel independent prognostic marker for gastric cancer. / Lin, Wen Chang; Li, Anna F Y; Chi, Chin Wen; Chung, Wen Wei; Huang, Chen Lung; Lui, Wing Yiu; Kung, Hsing-Jien; Wu, Chew Wun.

In: Clinical Cancer Research, Vol. 5, No. 7, 07.1999, p. 1745-1751.

Research output: Contribution to journalArticle

Lin, WC, Li, AFY, Chi, CW, Chung, WW, Huang, CL, Lui, WY, Kung, H-J & Wu, CW 1999, 'tie-1 protein tyrosine kinase: A novel independent prognostic marker for gastric cancer', Clinical Cancer Research, vol. 5, no. 7, pp. 1745-1751.
Lin WC, Li AFY, Chi CW, Chung WW, Huang CL, Lui WY et al. tie-1 protein tyrosine kinase: A novel independent prognostic marker for gastric cancer. Clinical Cancer Research. 1999 Jul;5(7):1745-1751.
Lin, Wen Chang ; Li, Anna F Y ; Chi, Chin Wen ; Chung, Wen Wei ; Huang, Chen Lung ; Lui, Wing Yiu ; Kung, Hsing-Jien ; Wu, Chew Wun. / tie-1 protein tyrosine kinase : A novel independent prognostic marker for gastric cancer. In: Clinical Cancer Research. 1999 ; Vol. 5, No. 7. pp. 1745-1751.
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