Tick-borne rickettsial pathogens in ticks and small mammals in Korea

Chul Min Kim, Ying Hua Yi, Do Hyeon Yu, Mi Jin Lee, Mae Rim Cho, Atul R. Desai, Smriti Shringi, Terry A. Klein, Heung Chul Kim, Jin Won Song, Luck Ju Baek, Sung Tae Chong, Monica L. O'Guinn, John S. Lee, In Yong Lee, Jin Ho Park, Janet E Foley, Joon Seok Chae

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Abstract

In order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (Hemaphysalis spp. and Ixodes spp.) were screened using molecular techniques. Ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the Republic of Korea (ROK) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). From the 1,638 tick samples, species-specific fragments of Anaplasma phagocytophilum (1 sample), Anaplasma platys (52 samples), Ehrlichia chaffeensis (29 samples), Ehrlichia ewingii (2 samples), Ehrlichia canis (18 samples), and Rickettsia rickettsii (28 samples) were amplified by PCR assay. Twenty-one pooled and individual tick samples had mixed infections of two (15 samples) or three (6 samples) pathogens. In addition, 424 spleen samples from small captured mammals (389 rodents, 33 insectivores, and 2 weasels) were screened for selected zoonotic pathogens. Species-specific DNA fragments of A. phagocytophilum (110 samples), A. platys (68 samples), E. chaffeensis (8 samples), E. ewingii (26 samples), E. canis (51 samples), and Rickettsia sp. (22 samples) were amplified by PCR assay. One hundred thirty small mammals had single infections, while 4, 14, and 21 striped field mice (Apodemus agrarius) had mixed infections of four, three, and two pathogens, respectively. Phylogenetic analysis based on nucleotide sequence comparison also revealed that Korean strains of E. chaffeensis clustered closely with those from China and the United States, while the Rickettsia (rOmpA) sequences clustered within a clade together with a Chinese strain. These results suggest that these agents should be considered in differential diagnosis while examining cases of acute febrile illnesses in humans as well as animals in the ROK.

Original languageEnglish (US)
Pages (from-to)5766-5776
Number of pages11
JournalApplied and Environmental Microbiology
Volume72
Issue number9
DOIs
StatePublished - Sep 2006

Fingerprint

tick
Ticks
Korea
small mammal
small mammals
Korean Peninsula
ticks
Mammals
pathogen
Ehrlichia chaffeensis
pathogens
Ehrlichia canis
Ehrlichia
Anaplasma phagocytophilum
Republic of Korea
sampling
Coinfection
Rickettsia rickettsii
Anaplasma
assay

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Kim, C. M., Yi, Y. H., Yu, D. H., Lee, M. J., Cho, M. R., Desai, A. R., ... Chae, J. S. (2006). Tick-borne rickettsial pathogens in ticks and small mammals in Korea. Applied and Environmental Microbiology, 72(9), 5766-5776. https://doi.org/10.1128/AEM.00431-06

Tick-borne rickettsial pathogens in ticks and small mammals in Korea. / Kim, Chul Min; Yi, Ying Hua; Yu, Do Hyeon; Lee, Mi Jin; Cho, Mae Rim; Desai, Atul R.; Shringi, Smriti; Klein, Terry A.; Kim, Heung Chul; Song, Jin Won; Baek, Luck Ju; Chong, Sung Tae; O'Guinn, Monica L.; Lee, John S.; Lee, In Yong; Park, Jin Ho; Foley, Janet E; Chae, Joon Seok.

In: Applied and Environmental Microbiology, Vol. 72, No. 9, 09.2006, p. 5766-5776.

Research output: Contribution to journalArticle

Kim, CM, Yi, YH, Yu, DH, Lee, MJ, Cho, MR, Desai, AR, Shringi, S, Klein, TA, Kim, HC, Song, JW, Baek, LJ, Chong, ST, O'Guinn, ML, Lee, JS, Lee, IY, Park, JH, Foley, JE & Chae, JS 2006, 'Tick-borne rickettsial pathogens in ticks and small mammals in Korea', Applied and Environmental Microbiology, vol. 72, no. 9, pp. 5766-5776. https://doi.org/10.1128/AEM.00431-06
Kim, Chul Min ; Yi, Ying Hua ; Yu, Do Hyeon ; Lee, Mi Jin ; Cho, Mae Rim ; Desai, Atul R. ; Shringi, Smriti ; Klein, Terry A. ; Kim, Heung Chul ; Song, Jin Won ; Baek, Luck Ju ; Chong, Sung Tae ; O'Guinn, Monica L. ; Lee, John S. ; Lee, In Yong ; Park, Jin Ho ; Foley, Janet E ; Chae, Joon Seok. / Tick-borne rickettsial pathogens in ticks and small mammals in Korea. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 9. pp. 5766-5776.
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