Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice

Yuichi Takeoka, Karen J. Whitmer, Shao Yuan Chen, Aftab A. Ansari, Richard L. Boyd, Leonard D. Shultz, Seishi Suehiro, M. Eric Gershwin

Research output: Contribution to journalArticle

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Abstract

Thymic maturation processes including MHC restriction and self-recognition require intimate association of thymocytes and stromal cells. Compared to the thymic architecture of various "normal" control strains of mice, defects in the thymic microenvironment have been demonstrated in New Zealand black (NZB) mice. Moreover, it is well known that NZB(H-dd) mice, when crossed with NZW(H-2u) mice, (NZB × NZW)F1, display a unique spectrum of autoimmune disease manifestations, including murine SLE. Using an extensive panel of monoclonal antibodies that define the thymic microenvironment, we examined two additional strains of (NZB × H-2u)F1 mice: (NZB × C57BL/10.PL)F1 and (NZB × PL/J)F1 mice to investigate the contributions of the H-2 and non-H-2 loci to the thymic abnormalities previously documented to occur in murine lupus. NZB, NZW, and (NZB × NZW)F1 mice were studied concurrently as were two additional control strains C57BL/6 and C57BL/10Sn. NZW mice had a normal thymic architecture as did the other H-2u mice and the control strains. In contrast, (NZB × NZW)F1 mice had a significantly altered thymic microenvironment; mild thymic abnormalities were also found in (NZB × PL/J)F1 but not in (NZB × C57BL/10.PL)F1. As expected, (NZB × NZW)F1 mice developed elevated titers of autoantibodies to DNA, proteinuria, and decreased life span. Interestingly, only (NZB × PL/J)F1 mice had increased levels of IgM antibodies to dsDNA, but did not manifest IgG anti-DNA or reduced survival. Defects in thymic stromal cells are associated directly to autoimmunity and their origin appears to be determined by non-H-2 loci.

Original languageEnglish (US)
Pages (from-to)297-307
Number of pages11
JournalClinical Immunology and Immunopathology
Volume76
Issue number3
DOIs
StatePublished - Sep 1995

Fingerprint

New Zealand
Epithelial Cells
Stromal Cells
DNA
Thymocytes
Autoimmunity
Proteinuria
Autoantibodies
Autoimmune Diseases
Immunoglobulin M
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Immunology

Cite this

Takeoka, Y., Whitmer, K. J., Chen, S. Y., Ansari, A. A., Boyd, R. L., Shultz, L. D., ... Gershwin, M. E. (1995). Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice. Clinical Immunology and Immunopathology, 76(3), 297-307. https://doi.org/10.1006/clin.1995.1129

Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice. / Takeoka, Yuichi; Whitmer, Karen J.; Chen, Shao Yuan; Ansari, Aftab A.; Boyd, Richard L.; Shultz, Leonard D.; Suehiro, Seishi; Gershwin, M. Eric.

In: Clinical Immunology and Immunopathology, Vol. 76, No. 3, 09.1995, p. 297-307.

Research output: Contribution to journalArticle

Takeoka, Y, Whitmer, KJ, Chen, SY, Ansari, AA, Boyd, RL, Shultz, LD, Suehiro, S & Gershwin, ME 1995, 'Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice', Clinical Immunology and Immunopathology, vol. 76, no. 3, pp. 297-307. https://doi.org/10.1006/clin.1995.1129
Takeoka Y, Whitmer KJ, Chen SY, Ansari AA, Boyd RL, Shultz LD et al. Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice. Clinical Immunology and Immunopathology. 1995 Sep;76(3):297-307. https://doi.org/10.1006/clin.1995.1129
Takeoka, Yuichi ; Whitmer, Karen J. ; Chen, Shao Yuan ; Ansari, Aftab A. ; Boyd, Richard L. ; Shultz, Leonard D. ; Suehiro, Seishi ; Gershwin, M. Eric. / Thymic Epithelial Cell Abnormalities in (NZB × H-2u)F1 Mice. In: Clinical Immunology and Immunopathology. 1995 ; Vol. 76, No. 3. pp. 297-307.
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