Thymic dependent anti hapten response in congenitally athymic (nude) mice immunized with DNP thymosin

A. Ahmed, A. H. Smith, K. W. Sell, M. Eric Gershwin, A. D. Steinberg, G. B. Thurman, A. L. Goldstein

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Immunization of congenitally athymic (nu/nu) and adult thymectomized, irradiated bone marrow, reconstituted (TxBm) mice with DNP 5-thymosin (dinitrophenylated-bovine thymosin fraction 5) was found to elicit IgM and IgG anti-DNP plaque-forming cells in these animals. Further studies indicated that this response was antigen specific and not due to polyclonal activation. Since the hormonal properties of the thymosin were retained following linkage with hapten and DNP-thymosin was immunogenic in CBA/N and (CBA/N female x DBA/2 male)F 1 male mice, animals previously shown to have an X-linked inability to respond to thymus-independent antigens, it was concluded that DNP-thymosin functions both as a hormone and as a T-dependent antigen in eliciting an immune response in nu/nu and TxBm mice. Additional support for this conclusion was provided by the demonstration that DNP-thymosin could specifically prime for and elicit an anamnestic response in nu/nu mice. These results indicate that further investigation of the immune activities of DNP-thymosin may provide valuable insight in characterizing the maturation of helper T cells and their subsequent interaction with B cells.

Original languageEnglish (US)
Pages (from-to)757-765
Number of pages9
Issue number5
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Immunology


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