Abstract
Using a monoclonal antibody raised against human platelet thrombospondin, we found anti-thrombospondin immunoreactivity in the extracellular matrix of avian embryos, coincident with the ventral pathways followed by trunk neural crest cells. To confirm that the antibody recognized thrombospondin-1 and to determine the tissue of origin of the thrombospondin matrix, a thrombospondin-1 cRNA probe was used for whole mount in situ hybridization. This probe revealed thrombospondin-1 mRNAs in the developing myotome before and during neural crest cell migration. The effect of thrombospondin-1 on neural crest cell migration, morphology, and adhesion was assayed in vitro. Quail trunk neural crest cells cultured on 4 μg/ml of thrombospondin-1 migrate at 1.14 ± 0.54 μm/min, which is significantly greater than the rate of cell migration on tissue culture plastic. Using a shaker-based adhesion assay, a significantly greater number of neural crest cells remain attached to dishes coated with 4 μg/ml of thrombospondin-1 than to tissue culture plastic alone. The number of neural crest cells that remain attached to 4 μg/ml of thrombospondin-1 is similar to the number that remain attached to dishes coated with 10 μg/ml of fibronectin. These observations indicate that neural crest cells migrate through a thrombospondin-filled extracellular matrix, and that thrombospondin-1 promotes neural crest cell migration and adhesion. Thus, thrombospondin-1 is the first somite-derived extracellular matrix molecule with properties consistent with a role in the promotion of migration into the anterior somite, as opposed to the repulsion of neural crest cells from the posterior half of the somite.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 312-322 |
| Number of pages | 11 |
| Journal | Developmental Dynamics |
| Volume | 214 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1999 |
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Keywords
- Extracellular matrix
- Neural crest
- Thrombospondin
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Thrombospondin-1 and neural crest cell migration. / Tucker, Richard P; Hagios, Carmen; Chiquet-Ehrismann, Ruth; Lawler, Jack; Hall, Ronelle J.; Erickson, Carol A.
In: Developmental Dynamics, Vol. 214, No. 4, 1999, p. 312-322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thrombospondin-1 and neural crest cell migration
AU - Tucker, Richard P
AU - Hagios, Carmen
AU - Chiquet-Ehrismann, Ruth
AU - Lawler, Jack
AU - Hall, Ronelle J.
AU - Erickson, Carol A.
PY - 1999
Y1 - 1999
N2 - Using a monoclonal antibody raised against human platelet thrombospondin, we found anti-thrombospondin immunoreactivity in the extracellular matrix of avian embryos, coincident with the ventral pathways followed by trunk neural crest cells. To confirm that the antibody recognized thrombospondin-1 and to determine the tissue of origin of the thrombospondin matrix, a thrombospondin-1 cRNA probe was used for whole mount in situ hybridization. This probe revealed thrombospondin-1 mRNAs in the developing myotome before and during neural crest cell migration. The effect of thrombospondin-1 on neural crest cell migration, morphology, and adhesion was assayed in vitro. Quail trunk neural crest cells cultured on 4 μg/ml of thrombospondin-1 migrate at 1.14 ± 0.54 μm/min, which is significantly greater than the rate of cell migration on tissue culture plastic. Using a shaker-based adhesion assay, a significantly greater number of neural crest cells remain attached to dishes coated with 4 μg/ml of thrombospondin-1 than to tissue culture plastic alone. The number of neural crest cells that remain attached to 4 μg/ml of thrombospondin-1 is similar to the number that remain attached to dishes coated with 10 μg/ml of fibronectin. These observations indicate that neural crest cells migrate through a thrombospondin-filled extracellular matrix, and that thrombospondin-1 promotes neural crest cell migration and adhesion. Thus, thrombospondin-1 is the first somite-derived extracellular matrix molecule with properties consistent with a role in the promotion of migration into the anterior somite, as opposed to the repulsion of neural crest cells from the posterior half of the somite.
AB - Using a monoclonal antibody raised against human platelet thrombospondin, we found anti-thrombospondin immunoreactivity in the extracellular matrix of avian embryos, coincident with the ventral pathways followed by trunk neural crest cells. To confirm that the antibody recognized thrombospondin-1 and to determine the tissue of origin of the thrombospondin matrix, a thrombospondin-1 cRNA probe was used for whole mount in situ hybridization. This probe revealed thrombospondin-1 mRNAs in the developing myotome before and during neural crest cell migration. The effect of thrombospondin-1 on neural crest cell migration, morphology, and adhesion was assayed in vitro. Quail trunk neural crest cells cultured on 4 μg/ml of thrombospondin-1 migrate at 1.14 ± 0.54 μm/min, which is significantly greater than the rate of cell migration on tissue culture plastic. Using a shaker-based adhesion assay, a significantly greater number of neural crest cells remain attached to dishes coated with 4 μg/ml of thrombospondin-1 than to tissue culture plastic alone. The number of neural crest cells that remain attached to 4 μg/ml of thrombospondin-1 is similar to the number that remain attached to dishes coated with 10 μg/ml of fibronectin. These observations indicate that neural crest cells migrate through a thrombospondin-filled extracellular matrix, and that thrombospondin-1 promotes neural crest cell migration and adhesion. Thus, thrombospondin-1 is the first somite-derived extracellular matrix molecule with properties consistent with a role in the promotion of migration into the anterior somite, as opposed to the repulsion of neural crest cells from the posterior half of the somite.
KW - Extracellular matrix
KW - Neural crest
KW - Thrombospondin
UR - http://www.scopus.com/inward/record.url?scp=0032955308&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032955308&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0177(199904)214:4<312::AID-AJA4>3.0.CO;2-A
DO - 10.1002/(SICI)1097-0177(199904)214:4<312::AID-AJA4>3.0.CO;2-A
M3 - Article
C2 - 10213387
AN - SCOPUS:0032955308
VL - 214
SP - 312
EP - 322
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 4
ER -