Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells

Anand S. Srivastava, Elena Nedelcu, Babak Esmaeli-Azad, Rangnath Mishra, Ewa Carrier

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The role of thrombopoietin (TPO) in adult hematopoiesis is well-established. A recent report suggests that TPO and vascular endothelial growth factor (VEGF) play a role in promoting formation of early erythropoietic progenitors in a nonhuman primate embryonic stem cell (ES) model. No such report exists for human ES cells as yet. Because TPO may become an important factor promoting human ES cell-derived hematopoiesis, we sought to investigate whether TPO in combination with VEGF can enhance human ES-derived hematopoiesis in an EB-derived culture system. The emphasis of this work was to demonstrate the molecular mechanisms involved in this process, specifically the role of c-mpl and its ligand TPO. Human ES cells were cultured to the EB state, and EB-derived secondary cultures supporting hematopoietic differentiation were established: condition 1, control (stem cell factor [SCF] and Flt3 ligand [Flt3L]); condition 2, SCF, Flt3L, and TPO; and condition 3, SCF, Flt3L, TPO, and VEGF. Cells were harvested daily, starting at day 2 and continuing until day 8, for reverse transcription-polymerase chain reaction and Western blot. There was no evidence of expression of c-mpl and VEGF receptor on the gene or protein level until day 8, when the formation of well-established hematopoietic colonies began. This correlated with the formation of CD34+/CD31- negative progenitors, mostly found in blast-forming units-erythroid-like colonies. We concluded that TPO and VEGF play an important synergistic role in the formation of early ES-derived hematopoietic progenitors that occurs through the c-mpl and VEGF receptors.

Original languageEnglish (US)
Pages (from-to)1456-1461
Number of pages6
JournalStem Cells
Volume25
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • CD31+ progenitors
  • CD34+ progenitors
  • Hematopoietic progenitors
  • Human embryonic stem cells

ASJC Scopus subject areas

  • Cell Biology

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    Srivastava, A. S., Nedelcu, E., Esmaeli-Azad, B., Mishra, R., & Carrier, E. (2007). Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells. Stem Cells, 25(6), 1456-1461. https://doi.org/10.1634/stemcells.2006-0701