Thrombolysis in a rabbit stroke model using liposomal-encapsulated streptokinase

Jonathan K Leach; E. A. O'Rear; Y. Miao; A. E. Johnson; E. S. Patterson

Thrombolysis in a rabbit stroke model using liposomal-encapsulated streptokinase

Jonathan K Leach, E. A. O'Rear, Y. Miao, A. E. Johnson, E. S. Patterson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Plasminogen activators have been used for years to treat thrombotic crises. While some have focused on the development of more fibrin-specific thrombolytics, we have chosen to examine alternative methods of delivering thrombolytic proteins to clots. Specifically, we and others have shown that plasminogen activators are more effective when encapsulated in liposomes than when infused as free proteins. In this study, we have explored the potential benefits of encapsulating streptokinase (SK) in the treatment of strokes by comparing the effects of free intravenous SK and liposomal-encapsulated SK (LESK). Following simulation of a stroke in a rabbit model, we infused comparable doses of either LESK or SK to determine the effect of each on reperfusion. LESK showed a significantly improved clot dissolving time over free SK [LESK: 19.3±12.1 min.; free SK: 74.3±41.4 min.]. Thus, the thrombolytic efficacy of SK is improved by encapsulation in liposomes for this experimental rabbit model, presumably by preventing premature inactivation of SK prior to LESK arrival and rupture at the clot.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of Publication	Piscataway, NJ, United States
Publisher	IEEE
Pages	776
Number of pages	1
Volume	2
ISBN (Print)	0780356756
State	Published - 1999
Externally published	Yes
Event	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999

Other

Other	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
City	Atlanta, GA, USA
Period	10/13/99 → 10/16/99

ASJC Scopus subject areas

Bioengineering

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Thrombolysis in a rabbit stroke model using liposomal-encapsulated streptokinase. / Leach, Jonathan K; O'Rear, E. A.; Miao, Y.; Johnson, A. E.; Patterson, E. S.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2 Piscataway, NJ, United States : IEEE, 1999. p. 776.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Leach, JK, O'Rear, EA, Miao, Y, Johnson, AE & Patterson, ES 1999, Thrombolysis in a rabbit stroke model using liposomal-encapsulated streptokinase. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 2, IEEE, Piscataway, NJ, United States, pp. 776, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.

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abstract = "Plasminogen activators have been used for years to treat thrombotic crises. While some have focused on the development of more fibrin-specific thrombolytics, we have chosen to examine alternative methods of delivering thrombolytic proteins to clots. Specifically, we and others have shown that plasminogen activators are more effective when encapsulated in liposomes than when infused as free proteins. In this study, we have explored the potential benefits of encapsulating streptokinase (SK) in the treatment of strokes by comparing the effects of free intravenous SK and liposomal-encapsulated SK (LESK). Following simulation of a stroke in a rabbit model, we infused comparable doses of either LESK or SK to determine the effect of each on reperfusion. LESK showed a significantly improved clot dissolving time over free SK [LESK: 19.3±12.1 min.; free SK: 74.3±41.4 min.]. Thus, the thrombolytic efficacy of SK is improved by encapsulation in liposomes for this experimental rabbit model, presumably by preventing premature inactivation of SK prior to LESK arrival and rupture at the clot.",

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AU - Patterson, E. S.

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N2 - Plasminogen activators have been used for years to treat thrombotic crises. While some have focused on the development of more fibrin-specific thrombolytics, we have chosen to examine alternative methods of delivering thrombolytic proteins to clots. Specifically, we and others have shown that plasminogen activators are more effective when encapsulated in liposomes than when infused as free proteins. In this study, we have explored the potential benefits of encapsulating streptokinase (SK) in the treatment of strokes by comparing the effects of free intravenous SK and liposomal-encapsulated SK (LESK). Following simulation of a stroke in a rabbit model, we infused comparable doses of either LESK or SK to determine the effect of each on reperfusion. LESK showed a significantly improved clot dissolving time over free SK [LESK: 19.3±12.1 min.; free SK: 74.3±41.4 min.]. Thus, the thrombolytic efficacy of SK is improved by encapsulation in liposomes for this experimental rabbit model, presumably by preventing premature inactivation of SK prior to LESK arrival and rupture at the clot.

AB - Plasminogen activators have been used for years to treat thrombotic crises. While some have focused on the development of more fibrin-specific thrombolytics, we have chosen to examine alternative methods of delivering thrombolytic proteins to clots. Specifically, we and others have shown that plasminogen activators are more effective when encapsulated in liposomes than when infused as free proteins. In this study, we have explored the potential benefits of encapsulating streptokinase (SK) in the treatment of strokes by comparing the effects of free intravenous SK and liposomal-encapsulated SK (LESK). Following simulation of a stroke in a rabbit model, we infused comparable doses of either LESK or SK to determine the effect of each on reperfusion. LESK showed a significantly improved clot dissolving time over free SK [LESK: 19.3±12.1 min.; free SK: 74.3±41.4 min.]. Thus, the thrombolytic efficacy of SK is improved by encapsulation in liposomes for this experimental rabbit model, presumably by preventing premature inactivation of SK prior to LESK arrival and rupture at the clot.

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